LOCUS       sequence-97610-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses eGFP with proK9 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK9_eGFP
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             326..348
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(386..414)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
       61 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
      121 ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc cacagctaac
      181 accacgtcgt ccctatctgc tgccctaggt ctatgagtgg ttgctggata actttaccgg
      241 catgcataag gctcgtaata tatattcagg gagaccacaa cggtttccct ctacaaataa
      301 ttttgtttaa ctttctagaa ataattttgt ttaactttaa gaaggagata tacatatgcg
      361 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      421 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
      481 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
      541 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
      601 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
      661 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
      721 aatcgag
//
