LOCUS       sequence-97612-e         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses eGFP with proK6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK6_eGFP
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             327..349
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(387..415)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
       61 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
      121 cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg ccacagctaa
      181 caccacgtcg tccctatctg ctgccctagg tctatgagtg gttgctggat aacttgtcgg
      241 gcatgcataa ggctcgtaat atatattcag ggagaccaca acggtttccc tctacaaata
      301 attttgttta actttctaga aataattttg tttaacttta agaaggagat atacatatgc
      361 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      421 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      481 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      541 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
      601 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
      661 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
      721 gaatcgag
//
