LOCUS       sequence-97616-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses eGFP with pro6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_pro6_eGFP
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(83..103)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     698..726
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtttgtata gttcatccat
       61 gccatgtgta atcccagcag ctgttacaaa ctcaagaagg accatgtggt ctctcttttc
      121 gttgggatct ttcgaaaggg cagattgtgt ggacaggtaa tggttgtctg gtaaaaggac
      181 agggccatcg ccaattggag tattttgttg ataatggtct gctagttgaa cgcttccatc
      241 ttcaatgttg tgtctaattt tgaagttaac tttgattcca ttcttttgtt tgtctgccat
      301 gatgtataca ttgtgtgagt tatagttgta ttccaatttg tgtccaagaa tgtttccatc
      361 ttctttaaaa tcaatacctt ttaactcgat tctattaaca agggtatcac cttcaaactt
      421 gacttcagca cgtgtcttgt agttcccgtc atctttgaaa aatatagttc tttcctgtac
      481 ataaccttcg ggcatggcac tcttgaaaaa gtcatgctgt ttcatatgat ctgggtatct
      541 cgcaaagcat tgaacaccat aaccgaaagt agtgacaagt gttggccatg gaacaggtag
      601 ttttccagta gtgcaaataa atttaagggt aagttttccg tatgttgcat caccttcacc
      661 ctctccactg acagaaaatt tgtgcccatt aacatcacca tctaattcaa caagaattgg
      721 gacaactcca gtg
//
