LOCUS       sequence-97618-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a fusion of full length CRY2 (E490G) with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Taslimi A, Vrana JD, Chen D, Borinskaya S, Mayer BJ, Kennedy MJ, 
            Tucker CL
  TITLE     An optimized optogenetic clustering tool for probing protein 
            interaction and function.
  JOURNAL   Nat Commun. 2014 Sep 18;5:4925. doi: 10.1038/ncomms5925.
  PUBMED    25233328
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-18"; volume: "5:4925. doi"; pages: " 
            10.1038/ncomms5925"
COMMENT     Sequence Label: CRY2olig(FL)-mCherry
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(129..839)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(407..426)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     676..694
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      836..845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
       61 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagagtcg
      121 cggccgcttt acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggcgcgt
      181 tcgtactgtt ccacgatggt gtagtcctcg ttgtgggagg tgatgtccaa cttgatgttg
      241 acgttgtagg cgccgggcag ctgcacgggc ttcttggcct tgtaggtggt cttgacctca
      301 gcgtcgtagt ggccgccgtc cttcagcttc agcctctgct tgatctcgcc cttcagggcg
      361 ccgtcctcgg ggtacatccg ctcggaggag gcctcccagc ccatggtctt cttctgcatt
      421 acggggccgt cggaggggaa gttggtgccg cgcagcttca ccttgtagat gaactcgccg
      481 tcctgcaggg aggagtcctg ggtcacggtc accacgccgc cgtcctcgaa gttcatcacg
      541 cgctcccact tgaagccctc ggggaaggac agcttcaagt agtcggggat gtcggcgggg
      601 tgcttcacgt aggccttgga gccgtacatg aactgagggg acaggatgtc ccaggcgaag
      661 ggcagggggc cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg
      721 ccctcgccct cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc
      781 ttgaagcgca tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcaccatg
      841 gtggcgaccg g
//
