LOCUS       sequence-97622-e         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively expresses eGFP with proK1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Bienick MS, Young KW, Klesmith JR, Detwiler EE, Tomek KJ, Whitehead 
            TA
  TITLE     The Interrelationship between Promoter Strength, Gene Expression, 
            and Growth Rate.
  JOURNAL   PLoS One. 2014 Oct 6;9(10):e109105. doi: 
            10.1371/journal.pone.0109105. eCollection 2014.
  PUBMED    25286161
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-10-6"; volume: "9(10):e109105. doi"; pages: " 
            10.1371/journal.pone.0109105. eCollection 2014"
COMMENT     Sequence Label: pJK_proK1_eGFP
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             334..356
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(394..422)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
       61 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
      121 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcca
      181 cagctaacac cacgtcgtcc ctatctgctg ccctaggtct atgagtggtt gctggataac
      241 tttactggca tgcataaggc tcgtaatata tattcaggga gaccacaacg gtttccctct
      301 acaaataatt ttgtttaact ttctagaaat aattttgttt aactttaaga aggagatata
      361 catatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      421 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      481 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      541 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      601 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      661 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      721 gttaatagaa tcgag
//
