LOCUS       sequence-97630-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector for sgRNA cloning (F+E modified backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: U6>sgRNA(F+E)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(26..45)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(27..45)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(132..149)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(383..402)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aagcggccat cagatctgcc ggactcccta tagtgagtcg tattaatttc gataagccag
       61 gttaacctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
      121 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
      181 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      241 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      301 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      361 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      421 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      481 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      541 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      601 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      661 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      721 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      781 accttc
//
