LOCUS       sequence-97631-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty vector for sgRNA cloning (F+E modified backbone).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: U6>sgRNA(F+E)
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
       61 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      121 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      181 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      241 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      301 tgataccgcg agatccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      361 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      421 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      481 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      541 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      601 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      661 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      721 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      781 cgtcaatac
//
