LOCUS       sequence-97634-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes the EGFP 
            gene to be recombined out of the construct, activating shRNA 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSico PGK GFP
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(45..544)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(93..112)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     486..509
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    586..619
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(637..657)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ttgctcacca tggtggcgac cgggagatct agatggatgc aggtcgaaag gcccggagat
       61 gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa tgccgggcct
      121 ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc gcccccggac
      181 ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta ttggccgctg
      241 ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac gagactagtg
      301 agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc gggggggaac
      361 ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa cggagccggt
      421 tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa
      481 gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag cgcctcccct
      541 acccggtaga atttcgaggt cgacgaattc aaaaaaccaa ggcttataac ttcgtataat
      601 ttatactata cgaagttata attactttac agttagggtg agtttccttt tgtgctgttt
      661 tttaaaataa taatttagta tttgtatctc ttatag
//
