LOCUS       sequence-97636-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-regulated lentiviral shRNA vector. Cre addition causes the EGFP 
            gene to be recombined out of the construct, activating shRNA 
            expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Ventura A, Meissner A, Dillon CP, McManus M, Sharp PA, Van Parijs L,
            Jaenisch R, Jacks T
  TITLE     Cre-lox-regulated conditional RNA interference from transgenes.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Jul 13. 101(28):10380-5.
  PUBMED    15240889
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2004 Jul 13. 101(28):10380-5."
COMMENT     Sequence Label: pSico PGK GFP
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     310..330
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(348..381)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     complement(458..481)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
       61 taccgggccc gctctagaga tccgacgcgc catctctagg cccgcgccgg ccccctcgca
      121 cagacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat ataatatttc
      181 ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct ttttaatact
      241 agctacattt tacatgatag gcttggattt ctataagaga tacaaatact aaattattat
      301 tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa agtaattata acttcgtata
      361 gtataaatta tacgaagtta taagccttgg ttttttgaat tcgtcgacct cgaaattcta
      421 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
      481 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
      541 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
      601 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
      661 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
//
