LOCUS       sequence-97639-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Howarth M, Liu W, Puthenveetil S, Zheng Y, Marshall LF, Schmidt MM, 
            Wittrup KD, Bawendi MG, Ting AY
  TITLE     Monovalent, reduced-size quantum dots for imaging receptors on 
            living cells.
  JOURNAL   Nat Methods. 2008 May . 5(5):397-9.
  PUBMED    18425138
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2008 May . 5(5):397-9."
COMMENT     Sequence Label: pDisplay-BirA-ER
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     160..219
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             223..249
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg
      121 gccgccagtg tgctggaatt cggcttgggg atatccacca tggagacaga cacactcctg
      181 ctatgggtac tgctgctctg ggttccaggt tccactggtg actatccata tgatgttcca
      241 gattatgctg gggcccagcc ggccagatct atgaaggata acaccgtgcc actgaaattg
      301 attgccctgt tagcgaacgg tgaatttcac tctggcgagc agttgggtga aacgctggga
      361 atgagccggg cggctattaa taaacacatt cagacactgc gtgactgggg cgttgatgtc
      421 tttaccgttc cgggtaaagg atacagcctg cctgagccta tccagttact taatgctaaa
      481 cagatattgg gtcagctgga tggcggtagt gtagccgtgc tgcctgtgat tgactccacg
      541 aatcagtacc ttcttgatcg tatcggagag cttaaatcgg gcgatgcttg cattgcagaa
      601 taccagcagg ctggccgtgg tcgccggggt cggaaatggt tttcgccttt tggcgcaaac
      661 ttatatttgt cgatgttctg gcgtctggaa caaggcccgg cggcggcgat tggtttaagt
      721 ctggttatcg gtatcgtgat ggcggaagta ttacgcaagc tgggtgcaga taaagttcgt
      781 gttaaatggc ctaatgacct ctatctgcag gatcgcaagc tggcaggcat tctggtggag
      841 ctgactggca aaactggcga tgcggcgcaa atagtcattg gagccgggat caacatggca
      901 atgcgccgtg ttgaagagag tgtcgttaat cagg
//
