LOCUS       sequence-97641-e        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter construct containing three hypoxia response 
            elements (24-mers) from the Pgk-1 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Emerling BM, Weinberg F, Liu JL, Mak TW, Chandel NS
  TITLE     PTEN regulates p300-dependent hypoxia-inducible factor 1 
            transcriptional activity through Forkhead transcription factor 3a 
            (FOXO3a).
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Feb 19. 105(7):2622-7.
  PUBMED    18268343
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2008 Feb 19. 105(7):2622-7."
COMMENT     Sequence Label: HRE-luciferase
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     intron          355..420
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             550..570
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
       61 aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaaatgta
      121 actgtattca gcgatgacga aattcttagc tattgtaata ctgcgatgag tggcagggcg
      181 gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc tacgcctgaa
      241 taagtgataa taagcggatg aatggcagaa attcgccgga tctttgtgaa ggaaccttac
      301 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
      361 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
      421 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
      481 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
      541 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
      601 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
      661 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
      721 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
      781 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
      841 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
      901 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
      961 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     1021 ttacaaataa agcaatagca tcac
//
