LOCUS       sequence-97647-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  dihydropterine reductase from human, pterin-4a-carbinolamine 
            dehydratase from human.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Satoh Y, Tajima K, Munekata M, Keasling JD, Lee TS
  TITLE     Engineering of L-tyrosine oxidation in Escherichia coli and 
            microbial production of hydroxytyrosol.
  JOURNAL   Metab Eng. 2012 Nov;14(6):603-10. doi: 10.1016/j.ymben.2012.08.002. 
            Epub 2012 Aug 29.
  PUBMED    22948011
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymben.2012.08.002"; journalName: "Metab Eng"; date: 
            "2012-11"; volume: "14"; issue: "6"; pages: "603-10"
COMMENT     Sequence Label: pBbE1k-2
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(373..378,379..395,396..402)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    410..426
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
       61 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
      121 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagcgcg aattgatctg
      181 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc
      241 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
      301 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
      361 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga
      421 taacaatttc agaattcaaa agatctaaga aggagatata catatggcag cagcagcagc
      481 agcaggtgaa gctcgtcgtg ttctggttta cggtggtcgt ggtgcactgg gcagccgctg
      541 cgttcaggca ttccgtgcgc gtaactggtg ggtggcttcc gtggacgttg tcgagaacga
      601 agaggcgtcc gcatccatta tcgttaaaat gaccgattcc ttcaccgaac aggcagatca
      661 ggttactgcc gaagttggca aactgctggg tgaagaaaaa gtagatgcga tcctgtgtgt
      721 tgctggtggc tgggctggtg gtaacgccaa aagcaaatcc ctgttcaaaa actgtgatct
      781 gatgtggaaa cagtctatct ggacctctac tatttctagc catctggcga ctaagcacct
      841 gaaagaaggt ggtctgctga ccctggccgg tgccaaagca gctctggatg gtacgccggg
      901 tatgattggt tacggcatgg ctaaaggcgc tgtacatcag ctgtgccagt ccctggcggg
      961 taaaaactct ggcatgccac cgggtgctgc ggcgatcgcg
//
