LOCUS       sequence-97648-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cleavage near e1350 locus in sqt-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS, Fire AZ
  TITLE     Efficient Marker-Free Recovery of Custom Genetic Modifications with 
            CRISPR/Cas9 in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Aug 26. pii: genetics.114.169730.
  PUBMED    25161212
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Aug 26. pii: genetics.114.169730."
COMMENT     Sequence Label: pJA54
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(62..164)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(165..196)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     244..260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     244..260
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_RNA        complement(403..478)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(499..846)
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
ORIGIN
        1 catccatgtt ggaatttaat cgcggcttca agcaagacgt ttcccgttga atatggctca
       61 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
      121 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaagtcaa aagcctccgg
      181 tcggaggctt ttgactttct gctatggagg tcaggtatga tttaaatggt cagtattgag
      241 cctcaggaaa cagctatgac atcaagctga ctagataatc tagctgatcg tggaccgatc
      301 atacgtataa tgccgtaaga tcacgggtcg cagcacagct cgcggtccag tagtgatcga
      361 cactgctcga tccgctcgca ccgctagcaa atttcacaaa aagcaccgac tcggtgccac
      421 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaacac
      481 gctaccccaa ctccacacaa gacatctcgc aataggaggt gtttatatag gggcaacaca
      541 agaggcggca gacatctcgt gagtttgaca gagtagtata aagagtgtgt gtgagagggt
      601 gtaggcattt cataacatat tttttgtgga ataattttgg aaaaaaggtg tttttttaat
      661 aatagctttt ataagagttt ctcaaaaatt gcatgctttt tttgaaaagg ttgacaaatt
      721 atagtagtac taaagttgct attttcaagt ctcagcagaa aattcacaac taaaaataaa
      781 atttgcaaaa cagagtgtgc gcctttaagg tgtacagttt tttattcctt tattgctagt
      841 tttttt
//
