LOCUS       sequence-97649-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cleavage near e1350 locus in sqt-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Arribere JA, Bell RT, Fu BX, Artiles KL, Hartman PS, Fire AZ
  TITLE     Efficient Marker-Free Recovery of Custom Genetic Modifications with 
            CRISPR/Cas9 in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Aug 26. pii: genetics.114.169730.
  PUBMED    25161212
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2014 Aug 26. pii: genetics.114.169730."
COMMENT     Sequence Label: pJA54
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..66
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     50..66
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        241..590
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans R07E5.16 U6 snRNA gene 
                     (Dickinson et al., 2013)"
     misc_RNA        611..686
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(829..845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(829..845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      893..924
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ggatctcaag aagatccttt gattttctac cgaagaaagg cccacccgtg taaaacgacg
       61 gccagtttat ctagtcagct tgattctagc tgatcgtgga ccggaaggtg agccagtgag
      121 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat gcgaccgccg
      181 gagggttgcg tttgagacgg gcgacagatc cagtcgcgct gctctcgtcg atccaagctt
      241 caaaaaaaac tagcaataaa ggaataaaaa actgtacacc ttaaaggcgc acactctgtt
      301 ttgcaaattt tatttttagt tgtgaatttt ctgctgagac ttgaaaatag caactttagt
      361 actactataa tttgtcaacc ttttcaaaaa aagcatgcaa tttttgagaa actcttataa
      421 aagctattat taaaaaaaca ccttttttcc aaaattattc cacaaaaaat atgttatgaa
      481 atgcctacac cctctcacac acactcttta tactactctg tcaaactcac gagatgtctg
      541 ccgcctcttg tgttgcccct atataaacac ctcctattgc gagatgtctt gtgtggagtt
      601 ggggtagcgt gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      661 ttgaaaaagt ggcaccgagt cggtgctttt tgtgaaattt gctagcggtg cgagcggatc
      721 gagcagtgtc gatcactact ggaccgcgag ctgtgctgcg acccgtgatc ttacggcatt
      781 atacgtatga tcggtccacg atcagctaga ttatctagtc agcttgatgt catagctgtt
      841 tcctgaggct caatactgac catttaaatc atacctgacc tccatagcag aaagtcaaaa
      901 gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact ttcaccataa
      961 tgaaataaga tcactaccgg gcgtatttt
//
