LOCUS       sequence-97650-e         733 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TFAM  No MTS L6 mutant in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Ngo HB, Lovely GA, Phillips R, Chan DC
  TITLE     Distinct structural features of TFAM drive mitochondrial DNA 
            packaging versus transcriptional activation.
  JOURNAL   Nat Commun. 2014;5:3077. doi: 10.1038/ncomms4077.
  PUBMED    24435062
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014"; volume: "5:3077. doi"; pages: " 
            10.1038/ncomms4077"
COMMENT     Sequence Label: Human_TFAM_NoMTS_L6_pET28a+
FEATURES             Location/Qualifiers
     source          1..733
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(1..77)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     283..302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     386..405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        386..404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    405..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             444..466
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             485..502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             512..529
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             533..565
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
       61 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
      121 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
      181 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      241 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      301 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      361 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
      421 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgggcag
      481 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggctag
      541 catgactggt ggacagcaaa tgggtcgcgg atccatgtca tctgtcttgg caagttgtcc
      601 aaagaaacct gtaagttctt accttcgatt ttctaaagaa caactaccca tatttaaagc
      661 tcagaaccca gatgcaaaaa ctacagaact aattagaaga attgcccagc gttggaggga
      721 acttcctgat tca
//
