LOCUS       sequence-97653-e         591 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TFAM  No MTS L6 mutant in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Ngo HB, Lovely GA, Phillips R, Chan DC
  TITLE     Distinct structural features of TFAM drive mitochondrial DNA 
            packaging versus transcriptional activation.
  JOURNAL   Nat Commun. 2014;5:3077. doi: 10.1038/ncomms4077.
  PUBMED    24435062
REFERENCE   2  (bases 1 to 591)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014"; volume: "5:3077. doi"; pages: " 
            10.1038/ncomms4077"
COMMENT     Sequence Label: Human_TFAM_NoMTS_L6_pET28a+
FEATURES             Location/Qualifiers
     source          1..591
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..49
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(102..119)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aagacccgtt tagaggcccc aaggggttat gctagttatt gctcagcggt ggcagcagcc
       61 aactcagctt cctttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc
      121 tcgagttaac actcctcagc accatatttt cgttgtttct ttattgtgcg acgtagaaga
      181 tcctttcgtc caacttcaat catttgttct tcccaagact tcatttcatt atgataacga
      241 gtttcgtcct ctttagcatg ctgaatatat aattcctttt cagagtcaga cagatttttc
      301 cagttttcct ttacagtctt cagcttttcc tgcggtgaat cacccttagc ttcttggaat
      361 ctttcagcta cataaacgtt ataagctgaa cgaggtcttt ttggttttcc aagcagtgtt
      421 aactctgctg cttttgtcat agctttcgct gctaaagctg cgtccatgat ttctttttcc
      481 aaagacataa tctgacttgg agttagctgt tctttaaatc tgcttatctc ttctttatat
      541 acctgccact ccgccctata agcatcttga tatatttttt tctttgaatc a
//
