LOCUS       sequence-97654-e         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TFAM  No MTS dimerization mutant in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Ngo HB, Lovely GA, Phillips R, Chan DC
  TITLE     Distinct structural features of TFAM drive mitochondrial DNA 
            packaging versus transcriptional activation.
  JOURNAL   Nat Commun. 2014;5:3077. doi: 10.1038/ncomms4077.
  PUBMED    24435062
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014"; volume: "5:3077. doi"; pages: " 
            10.1038/ncomms4077"
COMMENT     Sequence Label: Human _TFAM_NoMTS_Dimer_pET28a+
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..50
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(103..120)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
       61 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      121 ctcgagttaa cactcctcag caccatattt tcgttgtttc tttattgtgc gacgtagaag
      181 atcctttcgt ccaacttcaa tcatttgttc ttcccaagac ttcatttcat tatgataacg
      241 agtttcgtcc tctttagcat gctgaatata taattccttt tcagagtcag acagattttt
      301 ccagttttcc tttacagtct tcagcttttc ctgcggtgaa tcacccttag cttcttggaa
      361 tctttcagct acataaacgt tataagctga acgaggtctt tttggttttc caagcagtgt
      421 taactctttt ttttttgtca tagctttcct ttttaaatgt ttgtccatga tttctttttc
      481 caaagacata atctgacttg gagttagctg ttctttaaat gcgcttatct ctgctttata
      541 tacctgccac gccgccctat aagcatcttg aaatattttt ttcgctgaat caggaagttc
      601 cctccaacgc tgggcaattc ttctaattag ttctgtagtt tttgcatctg ggttctgagc
      661 tttaaatatg ggtagt
//
