LOCUS       sequence-97655-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TFAM  No MTS dimerization mutant in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Ngo HB, Lovely GA, Phillips R, Chan DC
  TITLE     Distinct structural features of TFAM drive mitochondrial DNA 
            packaging versus transcriptional activation.
  JOURNAL   Nat Commun. 2014;5:3077. doi: 10.1038/ncomms4077.
  PUBMED    24435062
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014"; volume: "5:3077. doi"; pages: " 
            10.1038/ncomms4077"
COMMENT     Sequence Label: Human _TFAM_NoMTS_Dimer_pET28a+
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     279..298
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     382..401
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        382..400
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    401..425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             440..462
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             481..498
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             508..525
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             529..561
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc
       61 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag
      121 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
      181 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
      241 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
      301 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
      361 gagatctcga tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca
      421 attcccctct agaaataatt ttgtttaact ttaagaagga gatataccat gggcagcagc
      481 catcatcatc atcatcacag cagcggcctg gtgccgcgcg gcagccatat ggctagcatg
      541 actggtggac agcaaatggg tcgcggatcc atgtcatctg tcttggcaag ttgtccaaag
      601 aaacctgtaa gttcttacct tcgattttct aaagaacaac tacccatatt taaagctcag
      661 aacccagatg caa
//
