LOCUS       sequence-97661-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral promoter-less Gateway destination vector, Puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pLenti X1 Puro DEST (694-6)
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      29..164
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    204..325
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(215..234)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     269..288
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             397..630
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctatagggcg aattgggtac gtcccgtagg cctccaaaaa agcctcctca ctacttctgg
       61 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
      121 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
      181 gggatagcta gagccagaca tgataagata cattgatgag tttggacaaa ccacaactag
      241 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
      301 cattataagc tgcaataaac aagttcctct cactctctga tattcatttc tttgcaagtt
      361 ataaatactg aataataaga tgacatgaac tactactgct agagattttc cacactgact
      421 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
      481 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
      541 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt
      601 gtcttcgttg ggagtgagct agcccttcca gtcccccctt ttcttttaaa aagtggctaa
      661 gatctacagc tgccttgtaa gtcattggtc ttaaaggtac cgggccctcg agatgcatgg
      721 ggtcgtgcgc tcctttcggt cgggc
//
