LOCUS       sequence-97669-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-DCatch encodes Dead streptavidin (negligible biotin binding) 
            bearing a C-terminal SpyCatcher, for generation of chimeric 
            SpyAvidin tetramers .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Dead Streptavidin-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..49)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             789..811
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    826..850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(850..869)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(851..869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcttcgggc tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg
       61 ccgcaagctt ttaaatatga gcgtcacctt tagttgcttt gccatttaca gtaacctgac
      121 cttgctcatt aactgtaaag gtaatagcag ttgctacctc ataaccgtct ggtgctgcgg
      181 tttcgacaaa tgtatatttt cctggataca ggtagaaatc tttcacttgt ccatctgaaa
      241 tccatgtact aatagtttta ccagatgaat cacgcaactc catagttgca ccagctaact
      301 ctttgccgtc ctcatcacgt tttgagaatt taatatgggt agcactatct cctgatcctg
      361 atcctgaagc agcggacggt ttaactttgg tgaaggtgtc gtgaccaacc agggtggatt
      421 tccacgcgtt agcttcggtg gtgccggagg tcaacaacca ctgggtgttg atacgagctt
      481 cagcaccacc aacgtactgg ccagaccagg tggtagcgga gtgagcgtta cggtagttgt
      541 ttttccaagc aacggtccaa cccagagcgg ttccggaacc gtcggtagcc ggagcggagt
      601 cgtaacgacc ggtcagaacg tatctagatt cagctccatc gtcatcaccg tcatcgtcac
      661 catcgtcatc accaacagcg gcttcgtagg taccggtcag agcaccgtca gcaccagcgg
      721 taacgatgaa ggtgtctccc agctgggcgt accaggtgcc ggtgatacca gcttcagcca
      781 tatgtatatc tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc
      841 tcacaattcc cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac
      901 gccggacgca tcgtggccgg catcaccggc gccac
//
