LOCUS       sequence-97670-e        1317 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-DCatch encodes Dead streptavidin (negligible biotin binding) 
            bearing a C-terminal SpyCatcher, for generation of chimeric 
            SpyAvidin tetramers .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 1317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Dead Streptavidin-SpyCatcher
FEATURES             Location/Qualifiers
     source          1..1317
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             790..812
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    827..851
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(851..870)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(852..870)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gap             1213..1230
                     /estimated_length=18
     gap             1239..1255
                     /estimated_length=17
     gap             1274..1284
                     /estimated_length=11
     gap             1290..1303
                     /estimated_length=14
ORIGIN
        1 tctcttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tttaaatatg agcgtcacct ttagttgctt tgccatttac agtaacctga
      121 ccttgctcat taactgtaaa ggtaatagca gttgctacct cataaccgtc tggtgctgcg
      181 gtttcgacaa atgtatattt tcctggatac aggtagaaat ctttcacttg tccatctgaa
      241 atccatgtac taatagtttt accagatgaa tcacgcaact ccatagttgc accagctaac
      301 tctttgccgt cctcatcacg ttttgagaat ttaatatggg tagcactatc tcctgatcct
      361 gatcctgaag cagcggacgg tttaactttg gtgaaggtgt cgtgaccaac cagggtggat
      421 ttccacgcgt tagcttcggt ggtgccggag gtcaacaacc actgggtgtt gatacgagct
      481 tcagcaccac caacgtactg gccagaccag gtggtagcgg agtgagcgtt acggtagttg
      541 tttttccaag caacggtcca acccagagcg gttccggaac cgtcggtagc cggagcggag
      601 tcgtaacgac cggtcagaac gtatctagat tcagctccat cgtcatcacc gtcatcgtca
      661 ccatcgtcat caccaacagc ggcttcgtag gtaccggtca gagcaccgtc agcaccagcg
      721 gtaacgatga aggtgtctcc cagctgggcg taccaggtgc cggtgatacc agcttcagcc
      781 atatgtatat ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg
      841 ctcacaattc ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta
      901 cgccggacgc atcgtggccg gcatcaccgg cgccacangn gcggttgctg gcgccnatat
      961 cgccgacntc accgatgggg anatcgggct cgccacttcn ngctnntgan cnntngttcg
     1021 gcgtgggtan nggngggcag gnccnnnnnn cgggggnnnt gttnggcgcc atcnncttgn
     1081 atgcncnntt nntgnnncgg cggtgntcaa cngncntcaa ccnnantnnc tgnnctgnnn
     1141 nntaatgcag nnnngcataa ggnaagannn nnnannnnnn cngannncnn ncnnnantgg
     1201 nncaaaaann ttnnnnnnnn nnnnnnnnnn ttnntnccnn nnnnnnnnnn nnnnngggng
     1261 gnnnnnnnnn aaannnnnnn nnnntnnnan nnnnnnnnnn nnnannnnnn nnncnng
//
