LOCUS       sequence-97672-e        1402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-DTag encodes Dead streptavidin (negligible biotin binding) 
            bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin 
            tetramers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Dead Streptavidin-SpyTag
FEATURES             Location/Qualifiers
     source          1..1402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..45)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             518..540
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    555..579
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(579..598)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(580..598)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(682..701)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        907..984
                     /gene="lacI"
                     /label=lacI promoter
     gap             1288..1300
                     /estimated_length=13
     gap             1338..1350
                     /estimated_length=13
     gap             1352..1361
                     /estimated_length=10
ORIGIN
        1 tncnnnnnnn ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gtgcggccgc
       61 aagcttttat tacgtaggtt tgtaggcatc caccatcacg atgtgggcgc cgcctgatcc
      121 tgatccggaa gcagcggacg gtttaacttt ggtgaaggtg tcgtgaccaa ccagggtgga
      181 tttccacgcg ttagcttcgg tggtgccgga ggtcaacaac cactgggtgt tgatacgagc
      241 ttcagcacca ccaacgtact ggccagacca ggtggtagcg gagtgagcgt tacggtagtt
      301 gtttttccaa gcaacggtcc aacccagagc ggttccggaa ccgtcggtag ccggagcgga
      361 gtcgtaacga ccggtcagaa cgtatctaga ttcagcgtta ccaacagcgg cttcgtaggt
      421 accggtcaga gcaccgtcag caccagcggt aacgatgaag gtgtctccca gctgggcgta
      481 ccaggtgccg gtgataccag cttcagccat atgtatatct ccttcttaaa gttaaacaaa
      541 attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag tcgtattaat
      601 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
      661 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
      721 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
      781 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
      841 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgag
      901 atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
      961 gagagtcaat ncagggnggt gaatgtgaaa ccagtaacgt ttatacgatg tcgcaganta
     1021 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaan caggncagcc acgttttctg
     1081 cgaaaacgcg ggaaaaagng gaagcngcga tggcggnanc tgaannacat ttcccnaccg
     1141 cgnggnanaa nnactggncg gggnnnacan nngttgctgn ntgnnttncc nccntncagt
     1201 nnnnnnccnn cacgcnncgn nncaaantnn cncggcgnnt taaanntnnn nncnatcann
     1261 ngggnnnnnn gcnnnnngnn nnnnangnnn nnnnnnnnnn cnnncnnnnn nntaagcggn
     1321 gnnncnnnat nntnnncnnn nnnnnnnnnn annnnnnnnn ntcnnntacn ntcnnncnna
     1381 tnnnnccngg nannnnnnnn nt
//
