LOCUS       sequence-97675-e         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-DTag encodes Dead streptavidin (negligible biotin binding) 
            bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin 
            tetramers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Dead Streptavidin-SpyTag
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             478..495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(548..566)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      562..609
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ggagatatac atatggctga agctggtata ccggacacct ggtacgccca gctgggagac
       61 accttcatcg ttaccgctgg tgctgacggt gctctgaccg gtacctacga agccgctgtt
      121 ggtaacgctg aatctagata cgttctgacc ggtcgttacg actccgctcc ggctaccgac
      181 ggttccggaa ccgctctggg ttggaccgtt gcttggaaaa acaactaccg taacgctcac
      241 tccgctacca cctggtctgg ccagtacgtt ggtggtgctg aagctcgtat caacacccag
      301 tggttgttga cctccggcac caccgaagct aacgcgtgga aatccaccct ggttggtcac
      361 gacaccttca ccaaagttaa accgtccgct gcttccggat caggatcagg cggcgcccac
      421 atcgtgatgg tggatgccta caaacctacg taataaaagc ttgcggccgc actcgagcac
      481 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
      541 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
      601 ggttttttgc tgaaaggagg aact
//
