LOCUS       sequence-97676-e        1414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET21-DTag encodes Dead streptavidin (negligible biotin binding) 
            bearing a C-terminal SpyTag, for generation of chimeric SpyAvidin 
            tetramers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Fairhead M, Veggiani G, Lever M, Yan J, Mesner D, Robinson CV, 
            Dushek O, van der Merwe PA, Howarth M
  TITLE     SpyAvidin hubs enable precise and ultrastable orthogonal 
            nanoassembly.
  JOURNAL   J Am Chem Soc. 2014 Sep 3;136(35):12355-63. doi: 10.1021/ja505584f. 
            Epub 2014 Aug 21.
  PUBMED    25111182
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja505584f";
            journalName: "J Am Chem Soc"; date: "2014-09-3- 3"; volume: "136"; 
            issue: "35"; pages: "12355-63"
COMMENT     Sequence Label: Dead Streptavidin-SpyTag
FEATURES             Location/Qualifiers
     source          1..1414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             505..522
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(575..593)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      589..636
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      673..1127
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(760..779)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     970..991
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gap             1361..1376
                     /estimated_length=16
ORIGIN
        1 ctannannnt ttgtttaact tnannaagga gatatacata tggctgaagc tggtataccg
       61 gacacctggt acgcccagct gggagacacc ttcatcgtta ccgctggtgc tgacggtgct
      121 ctgaccggta cctacgaagc cgctgttggt aacgctgaat ctagatacgt tctgaccggt
      181 cgttacgact ccgctccggc taccgacggt tccggaaccg ctctgggttg gaccgttgct
      241 tggaaaaaca actaccgtaa cgctcactcc gctaccacct ggtctggcca gtacgttggt
      301 ggtgctgaag ctcgtatcaa cacccagtgg ttgttgacct ccggcaccac cgaagctaac
      361 gcgtggaaat ccaccctggt tggtcacgac accttcacca aagttaaacc gtccgctgct
      421 tccggatcag gatcaggcgg cgcccacatc gtgatggtgg atgcctacaa acctacgtaa
      481 taaaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      541 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      601 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      661 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      721 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      781 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      841 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
      901 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
      961 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     1021 cttttgattt atanngnatt ttgccgattt cggcctattg gnnaaaaann gagctgattt
     1081 nacaaaaatt taacgcgaat tttaacaaat attaacgctt acaatttnnn ggcacttttc
     1141 ggggaaannn ncgcggaaac cctnatttgt ttattttttc nnnaatacnt tcaaatatgt
     1201 ancncntcat gannnnanna nnnnataaan gccttnnana anattgaaaa ggannannnn
     1261 nantnanttc nancatttcn ngnnnnccnn ntcccttttt nncngcnttt ttnncntnnn
     1321 nnnnttnnnt cnncccnnnn anncnggnnn annnnaanna nnnnnnnnnn nnnnnnggnn
     1381 nnnnnnangn gggnnntncn nnncnnnann nnna
//
