LOCUS       sequence-97690-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP7
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..25
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      54..63
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..86
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             99..125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    141..165
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tatcctccct ttatccagcc ctcactcctt ctctaggcgc cggaattaga tctgccacca
       61 tggactacaa ggatgacgat gacaagctcg atggaggata cccatacgat gttccagatt
      121 acgctgctcg aggttatcaa acaagtttgt acaaaaaagt tggcatgaac caccagcagc
      181 agcagcagca gcagcagaaa gcgggcgagc agcagttgag cgagcccgag gacatggagg
      241 tggaagcggg agacacagat gacccaccaa gaattactca gaaccctgtg atcaatggga
      301 atgtggccct gagtgatgga cacaacaccg cggaggagga catggaggat gacaccagtt
      361 ggcgctccga ggcaaccttt cagttcactg tggagcgctt cagcagactg agtgagtcgg
      421 tccttagccc tccgtgtttt gtgcgaaatc tgccatggaa gattatggtg atgccacgct
      481 tttatccaga cagaccacac caaaaaagcg taggattctt tctccagtgc aacgctgaat
      541 ctgattccgc gtcatggtct tgccatgcac aagcagtgct gaagataata aattacagag
      601 atgatgaaaa gtcgttcagt cgtcgtatta gtcatttgtt cttccataaa gaaaatgatt
      661 ggggattttc caattttatg gcctggagtg aagtgaccga tcctgagaaa ggatttatag
      721 atgatgacaa agttaccttt gaagtctttg tacaggcgga tgctccccat ggagttgcgt
      781 gggattcaaa gaagcacaca ggctacgtcg gcttaaagaa tcagggagcg acttgttaca
      841 tgaacagcct gctacagacg ttatttt
//
