LOCUS       sequence-97693-e        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Amphioxus GFPa1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Bomati EK, Haley JE, Noel JP, Deheyn DD
  TITLE     Spectral and structural comparison between bright and dim green 
            fluorescent proteins in Amphioxus.
  JOURNAL   Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469.
  PUBMED    24968921
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-27"; volume: "4:5469. doi"; pages: " 
            10.1038/srep05469"
COMMENT     Sequence Label: bfloGFPa1
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             29..51
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             70..87
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             97..114
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             778..795
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             820..837
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(890..908)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      904..951
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1075..1094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggttacggaa cgttcccctc tagaatattt tgtttaactt taagaaggag atataccatg
       61 ggcagcagcc atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatcat
      121 atgcctctgc ccgcaaccca cgacatccac cttcacggct ccatcaacgg ccacgagttc
      181 gacatggtgg ggggaggaaa aggcgacccg aacgccggct cgctggtgac cacagcgaaa
      241 tccaccaagg gtgccctgaa gttctctccc tacttgatga tcccccacct cgggtacggg
      301 tactaccagt acctccccta cccggacgga ccctcgcctt tccaggtctc catgttggaa
      361 ggatcggggt atgcagtcta ccgcgtgttc gactttgaag acggaggcaa gctgtctacc
      421 gagtttaagt actcctacga gggttcccat atcaaggccg acatgaagct gatgggaagc
      481 ggtttccctg acgacggccc agtcatgacc agccagattg tcgaccagga cggctgcgtg
      541 tccaagaaga cgtatcttaa caacaacacc atcgtggaca gcttcgactg gagttacaac
      601 ctgcagaatg ggaagcgcta cagggcccga gtgtcgagcc actacatctt cgacaagccc
      661 ttttcagccg atctcatgaa gaagcagccg gtcttcgttt accgcaagtg ccacgtgaag
      721 gctaccaaga ccgaagtcac cctggacgag agggagaagg cgttctacga gctggctcac
      781 catcaccatc accattagaa gcttgcggcc gcactcgagc accaccacca ccaccactga
      841 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
      901 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
      961 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     1021 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     1081 tcgctttctt cccttccttt ctcgccacgt tcgccgggtt ttccccgtaa agctctaaat
     1141 cggggggtcc ctttaagggt tccgaggtaa gggctttacg ggcccctcga cccccaaaaa
     1201 actttgattt agggggaagg ggttccccgt aagtggggcc attccccc
//
