LOCUS       sequence-97694-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Amphioxus GFPc1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Bomati EK, Haley JE, Noel JP, Deheyn DD
  TITLE     Spectral and structural comparison between bright and dim green 
            fluorescent proteins in Amphioxus.
  JOURNAL   Sci Rep. 2014 Jun 27;4:5469. doi: 10.1038/srep05469.
  PUBMED    24968921
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-06-27"; volume: "4:5469. doi"; pages: " 
            10.1038/srep05469"
COMMENT     Sequence Label: bfloGFPc1a
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             38..61
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             68..85
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             770..787
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(840..858)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      854..901
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(1025..1044)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 attttgttta actttaagaa ggagatatac catgaaacac caccaccacc accaccacca
       61 cggtggtctg gttccgcgtg gttcccatgg catgcctctt ccaacgactc acgaggtgca
      121 cgtctatggc tctatcaacg gtgttgagtt tgacttggtg ggtagcggca aaggcaaccc
      181 gaaggatggt tctgaggaga tccaagtgaa gtccactaag ggtcccctcg ggttctcccc
      241 gtacatcgtg gtccccaaca tcgggtacgg cttccaccag tacctgccct tcccagacgg
      301 gatgtcgcct ttccaggccg ctgcggacga tggctcgggg tacgtagtcc atcgtacgat
      361 tcagtttgaa gacggcgcct cgctgactgg caactaccga tattcctacg atggaggcca
      421 catcaaagga gagtttcatg tggttggtag cggtttccct gctgacggtc ccgtgatgac
      481 caaatcgctc acggctgtgg attggagcgt ggctaccatg ctcttcccaa acgacaccac
      541 cgttgtctcc accattgact ggacttgccc tactaccagc ggcaaacgct accatgccac
      601 gctgaggacc aactacacct tcgccaagcc gatagcggcc accatcctcc agaagcagcc
      661 gatgttcgtg ttccgtaaga cggaagtcaa ggcctctgac gcggagatca accttaagga
      721 gtggcagaag gctttccatg acctgtgaaa gcttgcggcc gcactcgagc accaccacca
      781 ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
      841 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
      901 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
      961 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1021 cccgctcctt tcgctttctt cccttcctt
//
