LOCUS       sequence-97695-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with mCherry ectodomain, no linker domain, G 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: mCherry_LD 0_CS G_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             166..399
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(475..496)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tgtgaaagtg ggtccgcgta cagccgcgcg cgtacgaaaa acaattacgg gtctaccatc
       61 gagggcctgc tcgatctccc ggacgacgac gcccccgaag aggcggggct ggcggctccg
      121 cgcctgtcct ttctccccgc gggacacacg cgcagactgt cgacggcccc cccgaccgat
      181 gtcagcctgg gggacgagct ccacttagac ggcgaggacg tggcgatggc gcatgccgac
      241 gcgctagacg atttcgatct ggacatgttg ggggacgggg attccccggg tccgggattt
      301 accccccacg actccgcccc ctacggcgct ctggatatgg ccgacttcga gtttgagcag
      361 atgtttaccg atgcccttgg aattgacgag tacggtggag gtaccggcgg aggctccggt
      421 ggtggctcta tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
      481 gagctggacg gcgacgtaaa cggccacaag ttcagcgtga ggggcgaggg cgagggcgat
      541 gccaccaacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      601 tggcccaccc tcgtgaccac cctgagccac ggcgtgcagt gcttcg
//
