LOCUS       sequence-97708-e        7714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the C-terminal fragment of split T7 RNAP split a position 
            179. Insert is driven by the arabinose inducible promoter PBAD. 
            Contains a constitutive araC ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Shis DL, Bennett MR
  TITLE     Library of synthetic transcriptional AND gates built with split T7 
            RNA polymerase mutants.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):5028-33. doi: 
            10.1073/pnas.1220157110. Epub 2013 Mar 11.
  PUBMED    23479654
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1220157110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-03-26- 26"; volume: "110"; issue: "13"; pages: "5028-33"
COMMENT     Sequence Label: pTSara-C
FEATURES             Location/Qualifiers
     source          1..7714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(96..974)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     1442..1461
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     primer_bind     complement(3757..3774)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3927..4013
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4105..4132
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4151..4242
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4696..5151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4783..4802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     CDS             complement(5706..6365)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6280..6299
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6366..6468)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(6860..6877)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6994..7539)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7074..7093)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7611..7630
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
       61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
      121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta
      181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
      241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
      301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag
      361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
      421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
      481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
      541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
      601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca
      661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
      721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
      781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
      841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
      901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
      961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
     1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta
     1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
     1141 aacaaaagtg tctataatca cggcagaaaa gtccctttga ttccattctt ttgtttgtct
     1201 gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc aagaatgttt
     1261 ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt atcaccttca
     1321 aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat agttctttcc
     1381 tgtacataac cttcgggcac tcgagtccac attgattatt tgcacggcgt cacactttgc
     1441 tatgccatag catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa
     1501 ctctctactg tttctccata cccgtttttt tgggctagca ctagtggagc cctatatata
     1561 aagatcaggg ccaggtatga aagcatttat gcaagttgtc gaggctgaca tgctctctaa
     1621 gggtctactc ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg
     1681 agtacgctgc atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa
     1741 tgctggcgta gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc
     1801 tatcgcaacc cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt
     1861 tcctcctaag ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc
     1921 tctggcgctg gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat
     1981 gcctgaggtg tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa
     2041 agtcctagcg gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc
     2101 tgcgattgag cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc
     2161 tctcaccgcg tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc
     2221 tcgccgtatc agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc
     2281 catctggttc ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa
     2341 cccgcaaggt aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg
     2401 taaggaaggt tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt
     2461 tccgttccct gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc
     2521 taagtctcca ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc
     2581 gttctgcttt gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc
     2641 gctggcgttt gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga
     2701 ggtaggtggt cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat
     2761 tgttgctaag aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga
     2821 agtagttacc gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac
     2881 taaggcactg gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc
     2941 agtcatgacg ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga
     3001 taccattcag ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc
     3061 tgctggatac atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt
     3121 tgaagcaatg aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa
     3181 gaagactgga gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt
     3241 ccctgtgtgg caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg
     3301 tcagttccgc ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa
     3361 acaggagtct ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa
     3421 gactgtagtg tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc
     3481 cttcggtacc attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt
     3541 tgacacatat gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt
     3601 gcacgagtct caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg
     3661 tgacatctta gagtcggact tcgcgttcgc gtaatctaga gtcgacctgc aggcatgcaa
     3721 gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta aatcagaacg
     3781 cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg tcccacctga
     3841 ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca
     3901 tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     3961 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgg
     4021 gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga cgcccgccat
     4081 aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt tttgcgtttc
     4141 tacaaactct tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4201 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4261 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4321 acgctggtga aagtaaaaga tgctgaagat cagttggggc aaactattaa ctggcgaact
     4381 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     4441 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4501 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4561 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4621 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4681 actttagatt gatttacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4981 cgttctttaa tagtggactc ttgttccaaa cttgaacaac actcaaccct atctcgggct
     5041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt taaaaggatc
     5161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5461 cctacatacc tcgctctgct aatcctgtta ccagtcaggc atttgagaag cacacggtca
     5521 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
     5581 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
     5641 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa
     5701 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     5761 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
     5821 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     5881 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     5941 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     6001 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     6061 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     6121 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     6181 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     6241 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     6301 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     6361 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     6421 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     6481 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
     6541 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     6601 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat
     6661 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg
     6721 acatcagcgc tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt
     6781 gaagtgcttc atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat
     6841 acaggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     6901 gagcggaaat ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta
     6961 acagggaagt gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa
     7021 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     7081 ccaggcgttt ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     7141 cggtgtcatt ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg
     7201 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct
     7261 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac
     7321 tggcagcagc cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa
     7381 ggctaaactg aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc
     7441 aaagagttgg tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt
     7501 tcagagcaag agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag
     7561 ataaaatatt tgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     7621 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     7681 gcgtagagga tctgctcatg tttgacagct tatc
//
