LOCUS       sequence-97727-e        5045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PIq driving expression of the FruR-L, the Fructose 
            inducible chimera with the wt Lac DBD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[FruR-L]
FEATURES             Location/Qualifiers
     source          1..5045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     973..992
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1012..1089)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1364..1595
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      1630..1716
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2251..3201)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3249..3471
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3963..4057)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4087..4878,4879..4947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4710..4729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4948..5039)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttag ctacggctga gcacgccgcg gcgatagaga ttacgtttaa tgcgcgttaa
       61 accaggtttt ggcttacgcg gttcgtccag gcttgccagg acaatctcca gcacacgctc
      121 tgcgacatcg cggtgacgtt gagccactgc cagcaccgga cactgtaaga agtcgagcag
      181 ttcgttatcg ccaaaggtgg caattgccag gtcagaaggc agtttgccgt cgcgacgcag
      241 cgtgacatcc atcactcctt gcaacaacgc aaacgacgtt gtgaacagcg cctgcggcat
      301 cggatgcgtt tccagccatt tttcgaataa ctgggcagcc gcctcccgct catagctgtt
      361 ggcatacagg aaatgcactt cgcgcggatc atctttccag gcagtacgga aaccttgttc
      421 acgcaggaag ctgacagaaa gctccggtag cgcaccaaga taaagcaccg tctcggcggg
      481 aaacttacgt aactcttccg ccagcatttc ggcatcatcc tgatcggcac caaccacgct
      541 ggtgaagtgt tcacgatcga gggcgcggtc cagcgcgaca atcgggaacg ggtcgttagc
      601 ccagcgttga taaaaaggat gctcaggagg caacgacgtc gaaacaataa tggcatcaac
      661 ctgacgctgt aaaaggtgct caatgcaccg catttcgttg tctggctgat cttctgagca
      721 ggcaatcagc agttgataac cccgttgccg cgcctggcgt tcaagatagt tagcgatgcg
      781 ggtatagctg gtgttctcca gatcggggat cacaagacca atagaacggc tacttttgcc
      841 cgccagttgt tgtgccacgc ggttgggaat gtaattgagc tccgccatcg ccgcttccac
      901 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
      961 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1021 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc
     1081 gatggtgtcc catgggcgca acgcaattaa tgtaagttag cgcgaattgt cgagggaaat
     1141 cataaaaaat ttatttgctt tcaggaaaat ttttctgtat aatagattca taaatttgag
     1201 agaagagttt aaatatggct ggttctcgca gaaagaaaca tatccatgaa atcccgcccc
     1261 gaattcatat gtctagatag ctgagtagcc tagagtgcgc gacaggtttg atgacaaaaa
     1321 attagcgcaa gaagacaaaa atcaccttgc gctaatgctc tgttacaggt cactaatacc
     1381 atctaagtag ttgattcata gtgactgcat atgttgtgtt ttacagtatt atgtagtctg
     1441 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     1501 agctttttta tactaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga
     1561 acaggtcact atcagtcaaa ataaaatcat tatttgattt caattttgtc ccactccccc
     1621 tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1681 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagggtacg
     1741 ggttttgctg cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga
     1801 tccggcttca ggtttgccgg ctgaaagcgc tatttcttcc agaattgcca tgattttttc
     1861 cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc
     1921 gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa caagttgtct caggtgttca
     1981 atttcatgtt ctagttgctt tgttttactg gtttcacctg ttctattagg tgttacatgc
     2041 tgttcatctg ttacattgtc gatctgttca tggtgaacag ctttaaatgc accaaaaact
     2101 cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt
     2161 tttccctttg atatctaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct
     2221 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt
     2281 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatgctt
     2341 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
     2401 agcatcgtgt agtgtttttc ttagtccgtt acgtaggtag gaatctgatg taatggttgt
     2461 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
     2521 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
     2581 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
     2641 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
     2701 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
     2761 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
     2821 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
     2881 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
     2941 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
     3001 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac
     3061 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
     3121 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
     3181 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
     3241 attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg
     3301 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
     3361 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
     3421 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
     3481 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
     3541 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
     3601 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
     3661 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga
     3721 ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt
     3781 ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct
     3841 ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat
     3901 gcacccagta aggcagcggt atcatcaaca ggcttacccg tcttactgtc cctagtgctt
     3961 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     4021 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcgt aaacttggtc
     4081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4201 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5041 agggg
//
