LOCUS       sequence-97728-e        5087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PIq driving expression of GalS-R, the Fucose inducible 
            chimera with the wt Lac DBD. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pzs1[GalS-L]
FEATURES             Location/Qualifiers
     source          1..5087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1015..1034
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1054..1131)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1406..1637
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      1672..1758
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2293..3243)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3291..3513
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4005..4099)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4129..4920,4921..4989))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4752..4771
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4990..5081)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttac atcgcctgat ttgttgagtt agtgatcgcc gccgcattct ggcgtgttgc
       61 gacagaatgg cgacgcacta acgtcggcat aaaacagtgg ctggcacgag gatcaatatt
      121 gcctgctgcc ccctgcaagg ccagttcggt ggctaatttc gccattgaag caatgggata
      181 acgcacggtc gttaattgcg ggtcggtgta acgggcgatg ggaatatcat cgaacccgat
      241 gattgagaga tgtaacggaa tcgcaatgcc attatctttt aatgctgtca gtgcgccagc
      301 ggccatattg tcgttataag caaatacagc ggtaagttgt agattgcgcc ccagcagttc
      361 aaccatcgcc gcctcaccgc ccggcatgtc cggcgtacca gcgccaatcc agctttccgg
      421 cggaataata tcctgctctt tcaacgcact catccagcct gctttacgca tggcgtcatc
      481 ttcaatgccg tggctggaag aaagataacc aatacgttga tgaccgttat tcagcaacat
      541 gcgcgtcgcc attcgggcac cgctgagatt atccaggcaa acgcaacgat gggcgtaccc
      601 cggcacaacg cggttgatta acaccatacc gggaatgtta tccataaatt gcgccagttc
      661 atcgtcactc aatgcttttg agtgaacaat caacgcatta caacgctggc gaattaacac
      721 ctcaatggcg tgacgctctt tttccgcttc atgatagcta ttgccgatta gcacgtattt
      781 ctgatgctgc tgagcgacca gatccaccgc ttttaccagc gcgccgaaaa acgcatcaga
      841 aacgtccatc accaccacgc caatggtgtc gctctgtttg cccgccagtt gggctgccac
      901 gcggttggga atgtaattga gctccgccat cgccgcttcc actttttccc gcgttttcgc
      961 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     1021 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     1081 cgggcgctat catgccatac cgcgaaaggt tttgcaccat tcgatggtgt cgctagcgcg
     1141 caacgcaatt aatgtaagtt agcgcgaatt gtcgagggaa atcataaaaa atttatttgc
     1201 tttcaggaaa atttttctgt ataatagatt cataaatttg agagaagagt ttaaatatgg
     1261 ctggttctcg cagaaagaaa catatccatg aaatcccgcc ccgaattcat atgtctagat
     1321 agctgagtag cctagagtgc gcgacaggtt tgatgacaaa aaattagcgc aagaagacaa
     1381 aaatcacctt gcgctaatgc tctgttacag gtcactaata ccatctaagt agttgattca
     1441 tagtgactgc atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     1501 atttaatata ttgatattta tatcatttta cgtttctcgt tcagcttttt tatactaagt
     1561 tggcattata aaaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca
     1621 aaataaaatc attatttgat ttcaattttg tcccactccc cctagaggca tcaaataaaa
     1681 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1741 ctcctgagta ggacaaatcc gccgccctag acctagggta cgggttttgc tgcccgcaaa
     1801 cgggctgttc tggtgttgct agtttgttat cagaatcgca gatccggctt caggtttgcc
     1861 ggctgaaagc gctatttctt ccagaattgc catgattttt tccccacggg aggcgtcact
     1921 ggctcccgtg ttgtcggcag ctttgattcg ataagcagca tcgcctgttt caggctgtct
     1981 atgtgtgact gttgagctgt aacaagttgt ctcaggtgtt caatttcatg ttctagttgc
     2041 tttgttttac tggtttcacc tgttctatta ggtgttacat gctgttcatc tgttacattg
     2101 tcgatctgtt catggtgaac agctttaaat gcaccaaaaa ctcgtaaaag ctctgatgta
     2161 tctatctttt ttacaccgtt ttcatctgtg catatggaca gttttccctt tgatatctaa
     2221 cggtgaacag ttgttctact tttgtttgtt agtcttgatg cttcactgat agatacaaga
     2281 gccataagaa cctcagatcc ttccgtattt agccagtatg ttctctagtg tggttcgttg
     2341 tttttgcgtg agccatgaga acgaaccatt gagatcatgc ttactttgca tgtcactcaa
     2401 aaattttgcc tcaaaactgg tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt
     2461 tcttagtccg ttacgtaggt aggaatctga tgtaatggtt gttggtattt tgtcaccatt
     2521 catttttatc tggttgttct caagttcggt tacgagatcc atttgtctat ctagttcaac
     2581 ttggaaaatc aacgtatcag tcgggcggcc tcgcttatca accaccaatt tcatattgct
     2641 gtaagtgttt aaatctttac ttattggttt caaaacccat tggttaagcc ttttaaactc
     2701 atggtagtta ttttcaagca ttaacatgaa cttaaattca tcaaggctaa tctctatatt
     2761 tgccttgtga gttttctttt gtgttagttc ttttaataac cactcataaa tcctcataga
     2821 gtatttgttt tcaaaagact taacatgttc cagattatat tttatgaatt tttttaactg
     2881 gaaaagataa ggcaatatct cttcactaaa aactaattct aatttttcgc ttgagaactt
     2941 ggcatagttt gtccactgga aaatctcaaa gcctttaacc aaaggattcc tgatttccac
     3001 agttctcgtc atcagctctc tggttgcttt agctaataca ccataagcat tttccctact
     3061 gatgttcatc atctgagcgt attggttata agtgaacgat accgtccgtt ctttccttgt
     3121 agggttttca atcgtggggt tgagtagtgc cacacagcat aaaattagct tggtttcatg
     3181 ctccgttaag tcatagcgac taatcgctag ttcatttgct ttgaaaacaa ctaattcaga
     3241 catacatctc aattggtcta ggtgatttta atcactatac caattgagat gggctagtca
     3301 atgataatta ctagtccttt tcctttgagt tgtgggtatc tgtaaattct gctagacctt
     3361 tgctggaaaa cttgtaaatt ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt
     3421 tttttttgtt tatattcaag tggttataat ttatagaata aagaaagaat aaaaaaagat
     3481 aaaaagaata gatcccagcc ctgtgtataa ctcactactt tagtcagttc cgcagtatta
     3541 caaaaggatg tcgcaaacgc tgtttgctcc tctacaaaac agaccttaaa accctaaagg
     3601 cttaagtagc accctcgcaa gctcgggcaa atcgctgaat attccttttg tctccgacca
     3661 tcaggcacct gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg
     3721 cagtgaatgg gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac
     3781 ccataataca agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat
     3841 gtggtgctat ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac
     3901 cacttcggat tatcccgtga caggtcattc agactggcta atgcacccag taaggcagcg
     3961 gtatcatcaa caggcttacc cgtcttactg tccctagtgc ttggattctc accaataaaa
     4021 aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact
     4081 ggatctatca acaggagtcc aagcgagctc gtaaacttgg tctgacagtt accaatgctt
     4141 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4201 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4261 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4321 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4381 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4441 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4501 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4561 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4621 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4681 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4741 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4801 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4861 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4921 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4981 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5041 gagcggatac atatttgaat gtatttagaa aaataaacaa atagggg
//
