LOCUS       sequence-97730-e        5253 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PI driving expression dimeric LacI, the Lactose inducible 
            repressor. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[LacI-L]
FEATURES             Location/Qualifiers
     source          1..5253
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     44..62
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        127..204
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(224..243)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1529..1760
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      1795..1881
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2416..3366)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3414..3636
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4128..4222)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4252..5043,5044..5112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4875..4894
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5113..5204)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gctagcctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
       61 gccctttcgt cttcacctcg acaattcgcg cgcgaaggcg aagcggcatg catttacgtt
      121 ggtaccgaca ccatcgaatg gcgcaaaacc tttcgcggta tggcatgata gcgcccggaa
      181 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      241 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      301 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      361 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      421 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      481 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      541 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      601 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      661 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      721 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      781 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      841 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      901 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
      961 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
     1021 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat
     1081 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1141 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1201 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtgaccatgg
     1261 gcgcaacgca attaatgtaa gttagcgcga attgtcgagg gaaatcataa aaaatttatt
     1321 tgctttcagg aaaatttttc tgtataatag attcataaat ttgagagaag agtttaaata
     1381 tggctggttc tcgcagaaag aaacatatcc atgaaatccc gccccgaatt catatgtcta
     1441 gatagctgag tagcctagag tgcgcgacag gtttgatgac aaaaaattag cgcaagaaga
     1501 caaaaatcac cttgcgctaa tgctctgtta caggtcacta ataccatcta agtagttgat
     1561 tcatagtgac tgcatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     1621 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt ttttatacta
     1681 agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     1741 tcaaaataaa atcattattt gatttcaatt ttgtcccact ccccctagag gcatcaaata
     1801 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1861 gctctcctga gtaggacaaa tccgccgccc tagacctagg gtacgggttt tgctgcccgc
     1921 aaacgggctg ttctggtgtt gctagtttgt tatcagaatc gcagatccgg cttcaggttt
     1981 gccggctgaa agcgctattt cttccagaat tgccatgatt ttttccccac gggaggcgtc
     2041 actggctccc gtgttgtcgg cagctttgat tcgataagca gcatcgcctg tttcaggctg
     2101 tctatgtgtg actgttgagc tgtaacaagt tgtctcaggt gttcaatttc atgttctagt
     2161 tgctttgttt tactggtttc acctgttcta ttaggtgtta catgctgttc atctgttaca
     2221 ttgtcgatct gttcatggtg aacagcttta aatgcaccaa aaactcgtaa aagctctgat
     2281 gtatctatct tttttacacc gttttcatct gtgcatatgg acagttttcc ctttgatatc
     2341 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca
     2401 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg
     2461 ttgtttttgc gtgagccatg agaacgaacc attgagatca tgcttacttt gcatgtcact
     2521 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt
     2581 ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc
     2641 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc
     2701 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt
     2761 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa
     2821 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat
     2881 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat
     2941 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa
     3001 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa
     3061 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc
     3121 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct
     3181 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct
     3241 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc
     3301 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc
     3361 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag
     3421 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac
     3481 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg
     3541 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa
     3601 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta
     3661 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa
     3721 aggcttaagt agcaccctcg caagctcggg caaatcgctg aatattcctt ttgtctccga
     3781 ccatcaggca cctgagtcgc tgtctttttc gtgacattca gttcgctgcg ctcacggctc
     3841 tggcagtgaa tgggggtaaa tggcactaca ggcgcctttt atggattcat gcaaggaaac
     3901 tacccataat acaagaaaag cccgtcacgg gcttctcagg gcgttttatg gcgggtctgc
     3961 tatgtggtgc tatctgactt tttgctgttc agcagttcct gccctctgat tttccagtct
     4021 gaccacttcg gattatcccg tgacaggtca ttcagactgg ctaatgcacc cagtaaggca
     4081 gcggtatcat caacaggctt acccgtctta ctgtccctag tgcttggatt ctcaccaata
     4141 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     4201 actggatcta tcaacaggag tccaagcgag ctcgtaaact tggtctgaca gttaccaatg
     4261 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4321 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4381 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4441 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4501 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4561 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4621 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4681 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4741 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4801 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4861 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4921 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4981 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     5041 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     5101 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     5161 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ctcgagttcc
     5221 gcgcacattt ccccgaaaag tgccacctga cgt
//
