LOCUS       sequence-97732-e        5039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PIq driving expression RbsR-T, the Ribose inducible chimera
            with the TAN DBD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[RbsR-T]
FEATURES             Location/Qualifiers
     source          1..5039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     967..986
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1006..1083)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1358..1589
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      1624..1710
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2245..3195)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3243..3465
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3957..4051)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4081..4872,4873..4941))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4704..4723
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4942..5033)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgagctaa gccgaaccgc gttccatcag aatcggagta agttgtaatc gttgttgctg
       61 aagggtcggc tgggttatcc gatggatgag tacatcaatc gccagctccc ccagttcatc
      121 tttcggttgg tggatagtgg ttaatggtgg cgtcataaag cttgccagtt cgatatcgtc
      181 atagccaatc accgcgatat cctgcggaac ctgtaactct gcctgatata acgcctggta
      241 aacgccaaca gccatagcgt catttccggt aaagacggcc tgaggacgca gcggatgtga
      301 tagcagttgg cgcatagcgt caaacccgcc gttaaattca aaatcaccag tgacttcata
      361 gccatcagga atgttgagac ccgcacgttt catcgccgcc cgataacctt ccaaccgcag
      421 gcgcgccgga gttttatcca gcgggccggt aatacaggcg atacgggtat gacctttatc
      481 gatcagatat tgcgttgcta agtctccgcc cagcaacgag ttatcctgaa taagatcgct
      541 gtcgccatcg aacggagccc agtccatcat cacagtaggc actgtcggat aacgttgcat
      601 gatttcacgc gaaggttgat gcgtttcggt gcacagtaac agcaagccat caacgcgttt
      661 ttgcatcagc gtttccagat tgcgattcat ccgctgttca tcgccttcgg tattgcaaag
      721 gacgagacta taaccgcgtt cgaagcagct gcgttcaacg ccacgcacca gttctgaata
      781 gaaaggattg gtactggcag tgatcaacat gccaatggta tggctcgctt tgcccgccag
      841 ttgttgtgcc acgcggttgg gaatgtaatt gagctccgcc atcgccgctt ccactttttc
      901 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg ttggaaacgg tcgccgtaga
      961 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     1021 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcacc attcgatggt
     1081 gtcccatggg cgcaacgcaa ttaatgtaag ttagcgcgaa ttgtcgaggg aaatcataaa
     1141 aaatttattt gctttcagga aaatttttct gtataataga ttcataaatt tgagagaaga
     1201 gtttaaatat ggctggttct cgcagaaaga aacatatcca tgaaatcccg ccccgaattc
     1261 atatgtctag atagctgagt agcctagagt gcgcgacagg tttgatgaca aaaaattagc
     1321 gcaagaagac aaaaatcacc ttgcgctaat gctctgttac aggtcactaa taccatctaa
     1381 gtagttgatt catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt
     1441 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     1501 tttatactaa gttggcatta taaaaaagca ttgcttatca atttgttgca acgaacaggt
     1561 cactatcagt caaaataaaa tcattatttg atttcaattt tgtcccactc cccctagagg
     1621 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1681 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctaggg tacgggtttt
     1741 gctgcccgca aacgggctgt tctggtgttg ctagtttgtt atcagaatcg cagatccggc
     1801 ttcaggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt tttccccacg
     1861 ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag catcgcctgt
     1921 ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg ttcaatttca
     1981 tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac atgctgttca
     2041 tctgttacat tgtcgatctg ttcatggtga acagctttaa atgcaccaaa aactcgtaaa
     2101 agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc
     2161 tttgatatct aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg
     2221 atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag
     2281 tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat gcttactttg
     2341 catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc
     2401 gtgtagtgtt tttcttagtc cgttacgtag gtaggaatct gatgtaatgg ttgttggtat
     2461 tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct
     2521 atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa
     2581 tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc attggttaag
     2641 ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt catcaaggct
     2701 aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata accactcata
     2761 aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat attttatgaa
     2821 tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt ctaatttttc
     2881 gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa ccaaaggatt
     2941 cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata caccataagc
     3001 attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg ataccgtccg
     3061 ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc ataaaattag
     3121 cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg ctttgaaaac
     3181 aactaattca gacatacatc tcaattggtc taggtgattt taatcactat accaattgag
     3241 atgggctagt caatgataat tactagtcct tttcctttga gttgtgggta tctgtaaatt
     3301 ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa attccgctag
     3361 acctttgtgt gttttttttg tttatattca agtggttata atttatagaa taaagaaaga
     3421 ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac tttagtcagt
     3481 tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa acagacctta
     3541 aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga atattccttt
     3601 tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag ttcgctgcgc
     3661 tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta tggattcatg
     3721 caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg cgttttatgg
     3781 cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg ccctctgatt
     3841 ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc taatgcaccc
     3901 agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt gcttggattc
     3961 tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct
     4021 gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgtaaactt ggtctgacag
     4081 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     4141 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     4201 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     4261 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     4321 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     4381 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     4441 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     4501 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     4561 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     4621 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     4681 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     4741 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4801 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4861 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4921 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4981 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaatagggg
//
