LOCUS       sequence-97736-e        4985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PI driving expression TreR-T, the Trehalose inducible 
            chimera with the TAN DBD..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[TreR-T]
FEATURES             Location/Qualifiers
     source          1..4985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     913..932
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(952..1029)
                     /gene="lacI"
                     /label=lacI promoter
     protein_bind    1304..1535
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      1570..1656
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2191..3141)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3189..3411
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(3903..3997)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4027..4818,4819..4887))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4650..4669
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4888..4979)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctcgagtcag gacagggtgg cggggatgat gatttgttgc ggttcgctgc gcccggttac
       61 ctgcgcgatc aactggcaag ccgcctggcg tccagcttcg gcgtaaccgg gatctacggt
      121 tacgatctcc ggatggagga atttcattaa cggcgtatta ccgacgctcg ccagttgcaa
      181 ggtgtcgatg cgttgctctt gcaggtattt acttgcgcca agtgccagcg tgtcggttgc
      241 gcacagtaag gcggtagttt caggcgtaat cacttttgca acgttctcat agccttgctt
      301 catagcaagc cctggcaggg cggcaacggg atgcagttta tgcgctttgc agaacgccag
      361 gtaggcttcg tgacgtcgct taccggttgt cacgtcactg tgcggcacgc cgagataact
      421 gatattacga tgcccctggt catacagccg ttgcatcagg attttgattg ccccttcgtc
      481 gtcataacag accgaagcaa agccttttgc gtcacgcgcc agcagaacca gcgatgactg
      541 ccagtgggct aacatttctt ctgttatgcc agtaaaaccg aacagcacta cgccgtcgat
      601 attacgccgt ttcagcactc ccaaatgttc ggcaactaat tgcggggaaa actgactttc
      661 catcatgatt gggtcgtaac cttgttcata gaacgctggc agcatggttt gaacggcgag
      721 attttctgac aacgaatcca gacgggtaac aatgatggcg accactttgc tctgtttgcc
      781 cgccagttgt tgtgcggcgc ggttgggaat gtaattgagc tccgccatcg ccgcttccac
      841 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgttgg aaacggtcgc
      901 cgtagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
      961 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1021 gatggtgtcc catgggcgca acgcaattaa tgtaagttag cgcgaattgt cgagggaaat
     1081 cataaaaaat ttatttgctt tcaggaaaat ttttctgtat aatagattca taaatttgag
     1141 agaagagttt aaatatggct ggttctcgca gaaagaaaca tatccatgaa atcccgcccc
     1201 gaattcatat gtctagatag ctgagtagcc tagagtgcgc gacaggtttg atgacaaaaa
     1261 attagcgcaa gaagacaaaa atcaccttgc gctaatgctc tgttacaggt cactaatacc
     1321 atctaagtag ttgattcata gtgactgcat atgttgtgtt ttacagtatt atgtagtctg
     1381 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc
     1441 agctttttta tactaagttg gcattataaa aaagcattgc ttatcaattt gttgcaacga
     1501 acaggtcact atcagtcaaa ataaaatcat tatttgattt caattttgtc ccactccccc
     1561 tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1621 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagggtacg
     1681 ggttttgctg cccgcaaacg ggctgttctg gtgttgctag tttgttatca gaatcgcaga
     1741 tccggcttca ggtttgccgg ctgaaagcgc tatttcttcc agaattgcca tgattttttc
     1801 cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct ttgattcgat aagcagcatc
     1861 gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa caagttgtct caggtgttca
     1921 atttcatgtt ctagttgctt tgttttactg gtttcacctg ttctattagg tgttacatgc
     1981 tgttcatctg ttacattgtc gatctgttca tggtgaacag ctttaaatgc accaaaaact
     2041 cgtaaaagct ctgatgtatc tatctttttt acaccgtttt catctgtgca tatggacagt
     2101 tttccctttg atatctaacg gtgaacagtt gttctacttt tgtttgttag tcttgatgct
     2161 tcactgatag atacaagagc cataagaacc tcagatcctt ccgtatttag ccagtatgtt
     2221 ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac gaaccattga gatcatgctt
     2281 actttgcatg tcactcaaaa attttgcctc aaaactggtg agctgaattt ttgcagttaa
     2341 agcatcgtgt agtgtttttc ttagtccgtt acgtaggtag gaatctgatg taatggttgt
     2401 tggtattttg tcaccattca tttttatctg gttgttctca agttcggtta cgagatccat
     2461 ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac
     2521 caccaatttc atattgctgt aagtgtttaa atctttactt attggtttca aaacccattg
     2581 gttaagcctt ttaaactcat ggtagttatt ttcaagcatt aacatgaact taaattcatc
     2641 aaggctaatc tctatatttg ccttgtgagt tttcttttgt gttagttctt ttaataacca
     2701 ctcataaatc ctcatagagt atttgttttc aaaagactta acatgttcca gattatattt
     2761 tatgaatttt tttaactgga aaagataagg caatatctct tcactaaaaa ctaattctaa
     2821 tttttcgctt gagaacttgg catagtttgt ccactggaaa atctcaaagc ctttaaccaa
     2881 aggattcctg atttccacag ttctcgtcat cagctctctg gttgctttag ctaatacacc
     2941 ataagcattt tccctactga tgttcatcat ctgagcgtat tggttataag tgaacgatac
     3001 cgtccgttct ttccttgtag ggttttcaat cgtggggttg agtagtgcca cacagcataa
     3061 aattagcttg gtttcatgct ccgttaagtc atagcgacta atcgctagtt catttgcttt
     3121 gaaaacaact aattcagaca tacatctcaa ttggtctagg tgattttaat cactatacca
     3181 attgagatgg gctagtcaat gataattact agtccttttc ctttgagttg tgggtatctg
     3241 taaattctgc tagacctttg ctggaaaact tgtaaattct gctagaccct ctgtaaattc
     3301 cgctagacct ttgtgtgttt tttttgttta tattcaagtg gttataattt atagaataaa
     3361 gaaagaataa aaaaagataa aaagaataga tcccagccct gtgtataact cactacttta
     3421 gtcagttccg cagtattaca aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag
     3481 accttaaaac cctaaaggct taagtagcac cctcgcaagc tcgggcaaat cgctgaatat
     3541 tccttttgtc tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg
     3601 ctgcgctcac ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga
     3661 ttcatgcaag gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt
     3721 ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct
     3781 ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat
     3841 gcacccagta aggcagcggt atcatcaaca ggcttacccg tcttactgtc cctagtgctt
     3901 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
     3961 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcgt aaacttggtc
     4021 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4081 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4141 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4201 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4261 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4321 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4381 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4441 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4501 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4561 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4621 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4681 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     4741 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     4801 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     4861 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     4921 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     4981 agggg
//
