LOCUS       sequence-97755-e        6290 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PIq driving expression GalS-T, and PIq driving expression 
            of RbsR-T..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[GalS-T,RbsR-T]
FEATURES             Location/Qualifiers
     source          1..6290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1048..1125)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     1169..1187
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1252..1329
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    2603..2834
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      2869..2955
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3490..4440)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4488..4710
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5202..5296)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5326..6117,6118..6186))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5949..5968
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6187..6278)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttacatcgcc tgatttgttg agttagtgat cgccgccgca ttctggcgtg ttgcgacaga
       61 atggcgacgc actaacgtcg gcataaaaca gtggctggca cgaggatcaa tattgcctgc
      121 tgccccctgc aaggccagtt cggtggctaa tttcgccatt gaagcaatgg gataacgcac
      181 ggtcgttaat tgcgggtcgg tgtaacgggc gatgggaata tcatcgaacc cgatgattga
      241 gagatgtaac ggaatcgcaa tgccattatc ttttaatgct gtcagtgcgc cagcggccat
      301 attgtcgtta taagcaaata cagcggtaag ttgtagattg cgccccagca gttcaaccat
      361 cgccgcctca ccgcccggca tgtccggcgt accagcgcca atccagcttt ccggcggaat
      421 aatatcctgc tctttcaacg cactcatcca gcctgcttta cgcatggcgt catcttcaat
      481 gccgtggctg gaagaaagat aaccaatacg ttgatgaccg ttattcagca acatgcgcgt
      541 cgccattcgg gcaccgctga gattatccag gcaaacgcaa cgatgggcgt accccggcac
      601 aacgcggttg attaacacca taccgggaat gttatccata aattgcgcca gttcatcgtc
      661 actcaatgct tttgagtgaa caatcaacgc attacaacgc tggcgaatta acacctcaat
      721 ggcgtgacgc tctttttccg cttcatgata gctattgccg attagcacgt atttctgatg
      781 ctgctgagcg accagatcca ccgcttttac cagcgcgccg aaaaacgcat cagaaacgtc
      841 catcaccacc acgccaatgg tgtcgctctg tttgcccgcc agttgggctg ccacgcggtt
      901 gggaatgtaa ttgagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac
      961 gtggctggcc tggttcacca cgttggaaac ggtcgccgta gagacaccgg catactctgc
     1021 gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg
     1081 ctatcatgcc ataccgcgaa aggttttgca ccattcgatg gtgtcgctag cctaagaaac
     1141 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     1201 cctcgacaat tcgcgcgcga aggcgaagcg gcatgcattt acgttggtac cgacaccatc
     1261 gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg
     1321 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctctacg
     1381 gcgaccgttt ccaacgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
     1441 gtggaagcgg cgatggcgga gctcaattac attcccaacc gcgtggcaca acaactggcg
     1501 ggcaaagcga gccataccat tggcatgttg atcactgcca gtaccaatcc tttctattca
     1561 gaactggtgc gtggcgttga acgcagctgc ttcgaacgcg gttatagtct cgtcctttgc
     1621 aataccgaag gcgatgaaca gcggatgaat cgcaatctgg aaacgctgat gcaaaaacgc
     1681 gttgatggct tgctgttact gtgcaccgaa acgcatcaac cttcgcgtga aatcatgcaa
     1741 cgttatccga cagtgcctac tgtgatgatg gactgggctc cgttcgatgg cgacagcgat
     1801 cttattcagg ataactcgtt gctgggcgga gacttagcaa cgcaatatct gatcgataaa
     1861 ggtcataccc gtatcgcctg tattaccggc ccgctggata aaactccggc gcgcctgcgg
     1921 ttggaaggtt atcgggcggc gatgaaacgt gcgggtctca acattcctga tggctatgaa
     1981 gtcactggtg attttgaatt taacggcggg tttgacgcta tgcgccaact gctatcacat
     2041 ccgctgcgtc ctcaggccgt ctttaccgga aatgacgcta tggctgttgg cgtttaccag
     2101 gcgttatatc aggcagagtt acaggttccg caggatatcg cggtgattgg ctatgacgat
     2161 atcgaactgg caagctttat gacgccacca ttaaccacta tccaccaacc gaaagatgaa
     2221 ctgggggagc tggcgattga tgtactcatc catcggataa cccagccgac ccttcagcaa
     2281 caacgattac aacttactcc gattctgatg gaacgcggtt cggcttagcc atgggcgcaa
     2341 cgcaattaat gtaagttagc gcgaattgtc gagggaaatc ataaaaaatt tatttgcttt
     2401 caggaaaatt tttctgtata atagattcat aaatttgaga gaagagttta aatatggctg
     2461 gttctcgcag aaagaaacat atccatgaaa tcccgccccg aattcatatg tctagatagc
     2521 tgagtagcct agagtgcgcg acaggtttga tgacaaaaaa ttagcgcaag aagacaaaaa
     2581 tcaccttgcg ctaatgctct gttacaggtc actaatacca tctaagtagt tgattcatag
     2641 tgactgcata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt
     2701 taatatattg atatttatat cattttacgt ttctcgttca gcttttttat actaagttgg
     2761 cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2821 taaaatcatt atttgatttc aattttgtcc cactccccct agaggcatca aataaaacga
     2881 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2941 ctgagtagga caaatccgcc gccctagacc tagggtacgg gttttgctgc ccgcaaacgg
     3001 gctgttctgg tgttgctagt ttgttatcag aatcgcagat ccggcttcag gtttgccggc
     3061 tgaaagcgct atttcttcca gaattgccat gattttttcc ccacgggagg cgtcactggc
     3121 tcccgtgttg tcggcagctt tgattcgata agcagcatcg cctgtttcag gctgtctatg
     3181 tgtgactgtt gagctgtaac aagttgtctc aggtgttcaa tttcatgttc tagttgcttt
     3241 gttttactgg tttcacctgt tctattaggt gttacatgct gttcatctgt tacattgtcg
     3301 atctgttcat ggtgaacagc tttaaatgca ccaaaaactc gtaaaagctc tgatgtatct
     3361 atctttttta caccgttttc atctgtgcat atggacagtt ttccctttga tatctaacgg
     3421 tgaacagttg ttctactttt gtttgttagt cttgatgctt cactgataga tacaagagcc
     3481 ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt
     3541 ttgcgtgagc catgagaacg aaccattgag atcatgctta ctttgcatgt cactcaaaaa
     3601 ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct
     3661 tagtccgtta cgtaggtagg aatctgatgt aatggttgtt ggtattttgt caccattcat
     3721 ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta gttcaacttg
     3781 gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca tattgctgta
     3841 agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt taaactcatg
     3901 gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct ctatatttgc
     3961 cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc tcatagagta
     4021 tttgttttca aaagacttaa catgttccag attatatttt atgaattttt ttaactggaa
     4081 aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg agaacttggc
     4141 atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga tttccacagt
     4201 tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt ccctactgat
     4261 gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt tccttgtagg
     4321 gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg tttcatgctc
     4381 cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta attcagacat
     4441 acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg ctagtcaatg
     4501 ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct agacctttgc
     4561 tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt
     4621 ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa aaaagataaa
     4681 aagaatagat cccagccctg tgtataactc actactttag tcagttccgc agtattacaa
     4741 aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt
     4801 aagtagcacc ctcgcaagct cgggcaaatc gctgaatatt ccttttgtct ccgaccatca
     4861 ggcacctgag tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag
     4921 tgaatggggg taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca
     4981 taatacaaga aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg
     5041 gtgctatctg actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac
     5101 ttcggattat cccgtgacag gtcattcaga ctggctaatg cacccagtaa ggcagcggta
     5161 tcatcaacag gcttacccgt cttactgtcc ctagtgcttg gattctcacc aataaaaaac
     5221 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     5281 tctatcaaca ggagtccaag cgagctcgta aacttggtct gacagttacc aatgcttaat
     5341 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5401 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5461 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5521 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5581 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     5641 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     5701 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     5761 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5821 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5881 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5941 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6001 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6061 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6121 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6181 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6241 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggctcgag
//
