LOCUS       sequence-97757-e        6222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PIq driving expression GalS-T, and PI driving expression of
            TreR-T..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZS1[GalS-T,TreR-T]
FEATURES             Location/Qualifiers
     source          1..6222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1053..1071
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1136..1213
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    2535..2766
                     /gene="attP"
                     /label=lambda attP
                     /bound_moiety="lambda integrase"
                     /note="integrase from phage lambda"
     terminator      2801..2887
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3422..4372)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4420..4642
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5134..5228)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5258..6049,6050..6118))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5881..5900
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6119..6210)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcaggacagg gtggcgggga tgatgatttg ttgcggttcg ctgcgcccgg ttacctgcgc
       61 gatcaactgg caagccgcct ggcgtccagc ttcggcgtaa ccgggatcta cggttacgat
      121 ctccggatgg aggaatttca ttaacggcgt attaccgacg ctcgccagtt gcaaggtgtc
      181 gatgcgttgc tcttgcaggt atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag
      241 taaggcggta gtttcaggcg taatcacttt tgcaacgttc tcatagcctt gcttcatagc
      301 aagccctggc agggcggcaa cgggatgcag tttatgcgct ttgcagaacg ccaggtaggc
      361 ttcgtgacgt cgcttaccgg ttgtcacgtc actgtgcggc acgccgagat aactgatatt
      421 acgatgcccc tggtcataca gccgttgcat caggattttg attgcccctt cgtcgtcata
      481 acagaccgaa gcaaagcctt ttgcgtcacg cgccagcaga accagcgatg actgccagtg
      541 ggctaacatt tcttctgtta tgccagtaaa accgaacagc actacgccgt cgatattacg
      601 ccgtttcagc actcccaaat gttcggcaac taattgcggg gaaaactgac tttccatcat
      661 gattgggtcg taaccttgtt catagaacgc tggcagcatg gtttgaacgg cgagattttc
      721 tgacaacgaa tccagacggg taacaatgat ggcgaccact ttgctctgtt tgcccgccag
      781 ttgttgtgcg gcgcggttgg gaatgtaatt gagctccgcc atcgccgctt ccactttttc
      841 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg ttggaaacgg tcgccgtaga
      901 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
      961 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcg ctagcctaag
     1021 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     1081 ttcacctcga caattcgcgc gcgaaggcga agcggcatgc atttacgttg gtaccgacac
     1141 catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt
     1201 cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
     1261 tacggcgacc gtttccaacg tggtgaacca ggccagccac gtttctgcga aaacgcggga
     1321 aaaagtggaa gcggcgatgg cggagctcaa ttacattccc aaccgcgtgg cagcccaact
     1381 ggcgggcaaa cagagcgaca ccattggcgt ggtggtgatg gacgtttctg atgcgttttt
     1441 cggcgcgctg gtaaaagcgg tggatctggt cgctcagcag catcagaaat acgtgctaat
     1501 cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt taattcgcca
     1561 gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac tggcgcaatt
     1621 tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt acgcccatcg
     1681 ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt tgctgaataa
     1741 cggtcatcaa cgtattggtt atctttcttc cagccacggc attgaagatg acgccatgcg
     1801 taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg aaagctggat
     1861 tggcgctggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac tgctggggcg
     1921 caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg gcgcactgac
     1981 agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg ggttcgatga
     2041 tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc ccattgcttc
     2101 aatggcgaaa ttagccaccg aactggcctt gcagggggca gcaggcaata ttgatcctcg
     2161 tgccagccac tgttttatgc cgacgttagt gcgtcgccat tctgtcgcaa cacgccagaa
     2221 tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccatgggcgc aacgcaatta
     2281 atgtaagtta gcgcgaattg tcgagggaaa tcataaaaaa tttatttgct ttcaggaaaa
     2341 tttttctgta taatagattc ataaatttga gagaagagtt taaatatggc tggttctcgc
     2401 agaaagaaac atatccatga aatcccgccc cgaattcata tgtctagata gctgagtagc
     2461 ctagagtgcg cgacaggttt gatgacaaaa aattagcgca agaagacaaa aatcaccttg
     2521 cgctaatgct ctgttacagg tcactaatac catctaagta gttgattcat agtgactgca
     2581 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
     2641 tgatatttat atcattttac gtttctcgtt cagctttttt atactaagtt ggcattataa
     2701 aaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     2761 ttatttgatt tcaattttgt cccactcccc ctagaggcat caaataaaac gaaaggctca
     2821 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     2881 gacaaatccg ccgccctaga cctagggtac gggttttgct gcccgcaaac gggctgttct
     2941 ggtgttgcta gtttgttatc agaatcgcag atccggcttc aggtttgccg gctgaaagcg
     3001 ctatttcttc cagaattgcc atgatttttt ccccacggga ggcgtcactg gctcccgtgt
     3061 tgtcggcagc tttgattcga taagcagcat cgcctgtttc aggctgtcta tgtgtgactg
     3121 ttgagctgta acaagttgtc tcaggtgttc aatttcatgt tctagttgct ttgttttact
     3181 ggtttcacct gttctattag gtgttacatg ctgttcatct gttacattgt cgatctgttc
     3241 atggtgaaca gctttaaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt
     3301 tacaccgttt tcatctgtgc atatggacag ttttcccttt gatatctaac ggtgaacagt
     3361 tgttctactt ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac
     3421 ctcagatcct tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga
     3481 gccatgagaa cgaaccattg agatcatgct tactttgcat gtcactcaaa aattttgcct
     3541 caaaactggt gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt
     3601 tacgtaggta ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct
     3661 ggttgttctc aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca
     3721 acgtatcagt cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta
     3781 aatctttact tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat
     3841 tttcaagcat taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag
     3901 ttttcttttg tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt
     3961 caaaagactt aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag
     4021 gcaatatctc ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg
     4081 tccactggaa aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca
     4141 tcagctctct ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca
     4201 tctgagcgta ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa
     4261 tcgtggggtt gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt
     4321 catagcgact aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca
     4381 attggtctag gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac
     4441 tagtcctttt cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac
     4501 ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt
     4561 atattcaagt ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag
     4621 atcccagccc tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt
     4681 cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca
     4741 ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg
     4801 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg
     4861 ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa
     4921 gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc
     4981 tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt
     5041 atcccgtgac aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac
     5101 aggcttaccc gtcttactgt ccctagtgct tggattctca ccaataaaaa acgcccggcg
     5161 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     5221 caggagtcca agcgagctcg taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5281 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5341 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5401 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5461 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5521 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5581 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5641 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5701 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5761 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5821 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5881 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5941 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6001 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6061 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6121 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6181 tatttgaatg tatttagaaa aataaacaaa taggggctcg ag
//
