LOCUS       sequence-97761-e        2908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains PTAN driving expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Shis DL, Hussain F, Meinhardt S, Swint-Kruse L, Bennett MR
  TITLE     Modular, Multi-Input Transcriptional Logic Gating with Orthogonal 
            LacI/GalR Family Chimeras.
  JOURNAL   ACS Synth Biol. 2014 Sep 19;3(9):645-51. doi: 10.1021/sb500262f. 
            Epub 2014 Jul 28.
  PUBMED    25035932
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500262f";
            journalName: "ACS Synth Biol"; date: "2014-09-19- 19"; volume: "3"; 
            issue: "9"; pages: "645-51"
COMMENT     Sequence Label: pZA2[PTAN:GFP]
FEATURES             Location/Qualifiers
     source          1..2908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             141..857
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      896..982
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(1018..1035)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1152..1697)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1232..1251)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1811..1905)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1936..2730)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2047..2066)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2657..2676
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 aataggcgta tcacgaggcc ctttcgtctt cacctcgaga attttaagcg gcttaaaatt
       61 gacattttaa gcgcttaaaa tgatactgag cacatcagca ggacgcactg accgaattca
      121 ttaaagagga gaaaggtacc atgtctaaag gtgaagaatt attcactggt gttgtcccaa
      181 ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg
      241 aaggtgatgc tacttacggt aaattgacct taaaatttat ttgtactact ggtaaattgc
      301 cagttccatg gccaacctta gtcactactt taacttatgg tgttcaatgt ttttctagat
      361 acccagatca tatgaaacaa catgactttt tcaagtctgc catgccagaa ggttatgttc
      421 aagaaagaac tatttttttc aaagatgacg gtaactacaa gaccagagct gaagtcaagt
      481 ttgaaggtga taccttagtt aatagaatcg aattaaaagg tattgatttt aaagaagatg
      541 gtaacatttt aggtcacaaa ttggaataca actataactc tcacaatgtt tacatcatgg
      601 ctgacaaaca aaagaatggt atcaaagtta acttcaaaat tagacacaac attgaagatg
      661 gttctgttca attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct
      721 tgttaccaga caaccattac ttatccactc aatctaaatt atccaaagat ccaaacgaaa
      781 agagagacca catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg
      841 atgaattgta caaatgaaag cttaattagc tgatctagac gcgtgctaga ggcatcaaat
      901 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      961 cgctctcctg agtaggacaa atccgccgcc ctagacctag gggatatatt ccgcttcctc
     1021 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg
     1081 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg
     1141 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca
     1201 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc
     1261 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     1321 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     1381 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg
     1441 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg
     1501 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt
     1561 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa
     1621 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca
     1681 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttc tagatttcag
     1741 tgcaatttat ctcttcaaat gtagcacctg aagtcagccc catacgatat aagttgttac
     1801 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1861 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctctcga
     1921 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     1981 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     2041 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     2101 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     2161 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga gcctggcgaa
     2221 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     2281 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     2341 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     2401 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     2461 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     2521 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     2581 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     2641 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     2701 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
     2761 agatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     2821 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tctaagaaac
     2881 cattattatc atgacattaa cctataaa
//
