LOCUS       sequence-97781-e         515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Eef1a (EF1alpha) promoter driving nls::Cas9::nls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: Eef1a-1955/-1>nls::Cas9::nls
FEATURES             Location/Qualifiers
     source          1..515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(120..140)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgccggtcgg tgttcccgag caccttgaac ttcttagagg gcaccttgta ctcgtcagtg
       61 atcacggccc acccgacgct attagtcccg atgtccagtc cgatagaata cttcttgtcc
      121 actttcctct tctttttggg gctagccatg gttgcggccg ctttggaagg ttggggttaa
      181 ccgaatgcac agaaatttgc tgtaaaataa gtttaagtta ttgttaaaaa gccaaaaggc
      241 gtatacaggg tatacttgct aagctaccag aactactaca gttaacgaac tgagtttttc
      301 agtaattcaa acgcctaacc agtactaaca tacttaaact aagtaaaacc taatgatttc
      361 atctttccta acaataaaaa tttaactaat caaactataa ttccttagag atgtactcag
      421 ataaaagata gaaccactca ccaaattgcc acagctcgaa aaggacaagt agaccgccga
      481 cacgtgagtc tttcgtaagt ctgaactgaa aaagg
//
