LOCUS       sequence-97784-e         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mesp driver driving nls::Cas9::nls.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: Mesp-1916/-1>nls::Cas9::nls
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             248..268
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aatgtgatta attacgaaaa tccagcgaat agaattgtca caacaagtca ttagcgacgg
       61 atatttcgcc tttgaaactt aaaggcgata atgactttgc ccgtttcatg cggcgataaa
      121 cgaactaatt agacacctcc tacagatata atggtaattc agaatcgtgt ggttatgtaa
      181 ttcgcaaaaa cattttaaca aaacaggttg atttgaaact tgtattgcgg ccgcaaccat
      241 ggctagcccc aaaaagaaga ggaaagtgga caagaagtat tctatcggac tggacatcgg
      301 gactaatagc gtcgggtggg ccgtgatcac tgacgagtac aaggtgccct ctaagaagtt
      361 caaggtgctc gggaacaccg accggcattc catcaagaaa aatctgatcg gagctctcct
      421 ctttgattca ggggagaccg ctgaagcaac ccgcctcaag cggactgcta gacggcggta
      481 caccaggagg aagaaccgga tttgttacct tcaagagata ttctccaacg aaatggcaaa
      541 ggtcgacgac agcttcttcc ataggctgga agaatcattc ctcgtggaag aggataagaa
      601 gcatgaacgg catcccatct tcggtaatat cgtcgacgag gtggcctatc acgagaaata
      661 cccaaccatc taccatcttc gcaaaaagct ggtggactca accgacaagg cagacctccg
      721 gcttatctac ctggccctgg cccacatgat caagttcaga
//
