LOCUS       sequence-97785-e        1070 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma 
            gondii SAG1 promotor. Vector also provides U6 controlled expression 
            of a single guide RNA for CRISPR disruption of TGME49_290860..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Behnke MS, Khan A, Sibley LD
  TITLE     Genetic mapping reveals that sinefungin resistance in Toxoplasma 
            gondii is controlled by a putative amino acid transporter locus that
            can be used as a negative selectable marker.
  JOURNAL   Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14.
  PUBMED    25480939
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell. 2014 Dec 5. pii: EC.00229-14."
COMMENT     Sequence Label: pSAG1::CAS9-U6::sg290860-6
FEATURES             Location/Qualifiers
     source          1..1070
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagttcgctc aggccgcctc tctcggcctt ggtcagattg tcgaacttcc tctgggtaat
       61 cagcttggca ttcagcagct ggcgccagta gttcttcatc ttcttcacga cctcttcgga
      121 gggcacgttg tcgctcttgc cccggttctt gtcgctccga gtcagcactt tgttatcgat
      181 ggagtcgtcc ttcagaaagc tctgaggcac gatatggtcc acatcgtagt cggacagccg
      241 gttgatgtcc agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta
      301 cagcttctcg ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc
      361 cagctctttg atgccctctt cgatccgctt cattctctcg cggctgttct tctgtccctt
      421 ctgggtggtc tggttctctc tggccatttc gatcacgatg ttctcgggct tgtgccggcc
      481 catcactttc acgagctcgt ccaccacctt cactgtctgc aggatgccct tcttaatggc
      541 ggggctgccg gccagattgg caatgtgctc gtgcaggcta tcgccctggc cggacacctg
      601 ggctttctgg atgtcctctt taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt
      661 tctgttggcg aagccgtcgg acttcaggaa atccaggatt gtcttgccgg actgcttgtc
      721 ccggatgccg ttgatcagct tccggctcag cctgccccag ccggtgtatc tccgccgctt
      781 cagctgcttc atcactttgt cgtcgaacag gtgggcatag gttttcagcc gttcctcgat
      841 catctctctg tcctcaaaca gtgtcagggt cagcacgata tcttccagaa tgtcctcgtt
      901 ttcctcattg tccaggaagt ccttgtcctt gataattttc agcagatcgt ggtatgtgcc
      961 cagggaggcg ttgaaccgat cttccacgcc ggagatttcc acggagtcga agcactcgat
     1021 tttcttgaag tagtcctctt tcagctgctt cacggtcact ttccggttgg
//
