LOCUS       sequence-97786-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma 
            gondii SAG1 promotor. Vector also provides U6 controlled expression 
            of a single guide RNA for CRISPR disruption of TGME49_290860..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Behnke MS, Khan A, Sibley LD
  TITLE     Genetic mapping reveals that sinefungin resistance in Toxoplasma 
            gondii is controlled by a putative amino acid transporter locus that
            can be used as a negative selectable marker.
  JOURNAL   Eukaryot Cell. 2014 Dec 5. pii: EC.00229-14.
  PUBMED    25480939
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryot 
            Cell. 2014 Dec 5. pii: EC.00229-14."
COMMENT     Sequence Label: pSAG1::CAS9-U6::sg290860-6
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagtctgtgg aagaagctgt cgtccacctt ggccatctcg ttgctgaaga tctcttgcag
       61 atagcagatc cggttcttcc gtctggtgta tcttcttctg gcggttctct tcagccgggt
      121 ggcctcggct gtttctccgc tgtcgaacag cagggcgccg atcaggttct tcttgatgct
      181 gtgccggtcg gtgttgccca gcaccttgaa tttcttgctg ggcaccttgt actcgtcggt
      241 gatcacggcc cagcccacag agttggtgcc gatgtccagg ccgatgctgt acttcttgtc
      301 cattttgtct agatgcatac tcgtcaaaaa accagaagaa ataatgaagt ggtgcagcga
      361 aaccgacata caaccgtgtg tttacacgac aatgacacat gtgacacaac cgagaagaac
      421 ggaaagctgc acaacggctc tgcccacaaa aactgtactc cggtcgtcgg cccttgccga
      481 gaacagtgag aatcagcagt gactacagct tcctacaggt gcacattgtg cggcagaaca
      541 cgcgtctcag ggtgcgtggt tagaacacgc gtctcagggt gcgtggttag aacacgcgtc
      601 tcagggtgcg tggttggaac acgcgtgtca gggtgcgtgg ttggaacacg cgtctcaagg
      661 tttgtggtta gaacacgcgt ctcgggagcc aaaggccagg aaggcgtgtc ccgcatccac
      721 cttctagacg catggtaaag ctcctcacga ccatgtggct aggtacgtgc tagcgctatg
      781 actacgaaac gttcacacgc ccgtcgcaca ttgttgccac tgtcaagaac ctgccctggt
      841 gttacgttgc ggcactgtga gcactgatag ggcaacgaga tcggccccta attcagcgag
      901 gccccagtac cgtgtcttgc agtgcatatt acctagacac cgtcagtttc aaatgcctcg
      961 tgtgggaagt ttcgttcgtt gtctcagact ttgcagtaga tagtcatcag caaggtgccc
     1021 accattcgac ctgtcactgc agtcttatta gtcgcaacga ccagtctttg aagctgc
//
