LOCUS       sequence-97792-e        4729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase reporter to measure miR-155 mediated differential 
            repression. Contains ATP2B1 3' UTR and mutated miR-155 sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Nam JW, Rissland OS, Koppstein D, Abreu-Goodger C, Jan CH, Agarwal 
            V, Yildirim MA, Rodriguez A, Bartel DP
  TITLE     Global analyses of the effect of different cellular contexts on 
            microRNA targeting.
  JOURNAL   Mol Cell. 2014 Mar 20;53(6):1031-43. doi: 
            10.1016/j.molcel.2014.02.013. Epub 2014 Mar 13.
  PUBMED    24631284
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.02.013"; journalName: "Mol Cell"; date: 
            "2014-03-20- 20"; volume: "53"; issue: "6"; pages: "1031-43"
COMMENT     Sequence Label: pIS1 mATP2B1
FEATURES             Location/Qualifiers
     source          1..4729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1608..1729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1619..1638)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1673..1692
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1862..1966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1967..2035,2036..2827)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2185..2204)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2998..3586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3487..3506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3661..4412
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     intron          4482..4614
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4597..4616
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4659..4677
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4687..4729,1..893)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
ORIGIN
        1 taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat tcatttatta
       61 attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat ggtaacgcgg
      121 cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg cggtgtatta
      181 taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct tataggttac
      241 ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca aagaagatca
      301 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag
      361 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg
      421 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg
      481 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag
      541 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc
      601 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac
      661 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta
      721 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta
      781 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa
      841 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taattctagg
      901 agctctatac cggtctcgat atcactacta gtgttctaga gggtggtgga ctatcttgaa
      961 gaaccctttc aactctaaaa attgacccta tcaactggag agcccacaag gcatagggaa
     1021 tagagaaaag ggaaatgttt tcagttttct tttttatttt ggttatgctt aaatatgaat
     1081 gtaattacca aaagatttag aagtgcatgt gctttggagg atttgtattg agcttttaca
     1141 gtattcattt ttcaactcaa ggcaatggct ttctacacca actctaatcc ataaacgggt
     1201 cttatgacat ctatgaagta gtagcaagac atgcttagtg tgtatttctc tctttgagac
     1261 actgtaattt ctaccagaaa tttccagaac gtaatgtagg tagaaaaaaa tgcaagcaag
     1321 ctgttaaaga tcttggatcc cattatatag tatgtatagc tgaaatctgt aattcaatca
     1381 ctttttctct tttatcctct aaccaaaaaa ttgtttaatt ttgcatccca aatgttttta
     1441 atctttgtat attttttaaa aatccttttc tcctcatcat tgcctttttt gtggttgtaa
     1501 atagacttac ttgcactttg aagatgagtt actccttgtc atcttacaaa tatgtgatat
     1561 ggtaattttc ataacagatg tcaggcggcc gcttcgagca gacatgataa gatacattga
     1621 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     1681 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     1741 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     1801 aaacctctac aaatgtggta aaatcgataa ggatccaggt ggcacttttc ggggaaatgt
     1861 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1921 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1981 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2041 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2101 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2161 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2221 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2281 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2341 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2401 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2461 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2521 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2581 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2641 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     2701 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     2761 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     2821 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     2881 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2941 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3001 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3061 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3121 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     3181 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3241 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3301 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3361 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3421 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3481 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3541 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3601 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatggctc gacagatcta
     3661 aatgagtctt cggacctcgc gggggccgct taagcggtgg ttagggtttg tctgacgcgg
     3721 ggggaggggg aaggaacgaa acactctcat tcggaggcgg ctcggggttt ggtcttggtg
     3781 gccacgggca cgcagaagag cgccgcgatc ctcttaagca cccccccgcc ctccgtggag
     3841 gcgggggttt ggtcggcggg tggtaactgg cgggccgctg actcgggcgg gtcgcgcgcc
     3901 ccagagtgtg accttttcgg tctgctcgca gacccccggg cggcgccgcc gcggcggcga
     3961 cgggctcgct gggtcctagg ctccatgggg accgtatacg tggacaggct ctggagcatc
     4021 cgcacgactg cggtgatatt accggagacc ttctgcggga cgagccgggt cacgcggctg
     4081 acgcggagcg tccgttgggc gacaaacacc aggacggggc acaggtacac tatcttgtca
     4141 cccggaggcg cgagggactg caggagcttc agggagtggc gcagctgctt catccccgtg
     4201 gcccgttgct cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt
     4261 tctatgatga cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg
     4321 cagtcggggc ggcgcggtcc caggtccact tcgcatatta aggtgacgcg tgtggcctcg
     4381 aacaccgagc gaccctgcag cgacccgctt aaaagcttga ttcttctgac acaacagtct
     4441 cgaacttaag ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa
     4501 gacaggttta aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt
     4561 tctgataggc acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc
     4621 actcccagtt caattacagc tcttaaggct agagtactta atacgactca ctataggcta
     4681 gccaccatga cttcgaaagt ttatgatcca gaacaaagga aacggatga
//
