LOCUS       sequence-97794-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter driving Ebf.774 (F+E) sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: U6>Ebf.774(F+E)
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(24..43)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(25..43)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(130..147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(381..400)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccatca gatctgccgg actccctata gtgagtcgta ttaatttcga taagccaggt
       61 taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
      121 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      181 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      241 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      301 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      361 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      421 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      481 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      541 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      601 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      661 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      721 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      781 cttcgg
//
