LOCUS       sequence-97796-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter driving Ebf.774 (F+E) sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: U6>Ebf.774(F+E)
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     786..805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
       61 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
      121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
      181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
      241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
      301 gcgagatcca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
      361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
      421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
      481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
      541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
      601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
      661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
      721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
      781 acgggataat accgcgccac atagcag
//
