LOCUS       sequence-97800-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6 promoter driving control (F+E) sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Stolfi A, Gandhi S, Salek F, Christiaen L
  TITLE     Tissue-specific genome editing in Ciona embryos by CRISPR/Cas9.
  JOURNAL   Development. 2014 Nov;141(21):4115-20. doi: 10.1242/dev.114488.
  PUBMED    25336740
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1242/dev"; 
            journalName: "Development"; date: "2014-11"; volume: "141"; issue: 
            "21"; pages: "4115-20"
COMMENT     Sequence Label: U6>Control(F+E)
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     791..810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
       61 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      121 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      181 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      241 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
      301 ataccgcgag atccacgctc accggctcca gatttatcag caataaacca gccagccgga
      361 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      421 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      481 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      541 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      601 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
      661 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
      721 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
      781 tcaatacggg ataataccgc gccacatagc agaac
//
