LOCUS       sequence-97823-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Fujinaga K, Taube R, Wimmer J, Cujec TP, Peterlin BM
  TITLE     Interactions between human cyclin T, Tat, and the transactivation 
            response element (TAR) are disrupted by a cysteine to tyrosine 
            substitution found in mouse cyclin T.
  JOURNAL   Proc Natl Acad Sci U S A. 1999 Feb 16. 96(4):1285-90.
  PUBMED    9990016
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1999 Feb 16. 96(4):1285-90."
COMMENT     Sequence Label: pGEX-2TK cyclin T1 (1-300) (P#432)
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     80..102
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     94..111
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     95..111
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(127..143)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(127..143)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(140..162)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    151..167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(175..181,182..199,200..205))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
       61 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaatccgta
      121 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      181 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aacttacatt
      241 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      301 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc
      361 ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt
      421 gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta
      481 acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg
      541 caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt
      601 tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa
      661 aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag
      721 tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg
      781 ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgc
//
