LOCUS       sequence-97848-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T
  TITLE     Human Argonaute2 mediates RNA cleavage targeted by miRNAs and 
            siRNAs.
  JOURNAL   Mol Cell. 2004 Jul 23. 15(2):185-97.
  PUBMED    15260970
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jul 23. 15(2):185-97."
COMMENT     Sequence Label: pIRESneo-FLAG/HA Ago2 corrected
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             129..152
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             153..179
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc ttggtaccga gctcggatcg atatcgccgg
      121 ccgccatgga ctacaaggac gacgatgaca agtaccctta tgacgtgccc gattacgcta
      181 gcggccgcat gtactcggga gccggccccg cacttgcacc tcctgcgccg ccgcccccca
      241 tccaaggata tgccttcaag cctccaccta gacccgactt tgggacctcc gggagaacaa
      301 tcaaattaca ggccaatttc ttcgaaatgg acatccccaa aattgacatc tatcattatg
      361 aattggatat caagccagag aagtgcccga ggagagttaa cagggaaatc gtggaacaca
      421 tggtccagca ctttaaaaca cagatctttg gggatcggaa gcccgtgttt gacggcagga
      481 agaatctata cacagccatg ccccttccga ttgggaggga caaggtggag ctggaggtca
      541 cgctgccagg agaaggcaag gatcgcatct tcaaggtgtc catcaagtgg gtgtcctgcg
      601 tgagcttgca ggcgttacac gatgcacttt cagggcggct gcccagcgtc ccttttgaga
      661 cgatccaggc cctggacgtg gtcatgaggc acttgccatc catgaggtac acccccgtgg
      721 gccgctcctt cttcaccgcg tccgaaggct gctctaaccc tcttggcggg ggccgagaag
      781 tgtggtttgg cttccatcag tccgtccggc cttctctctg gaaaatgatg ctgaatattg
      841 atgtgtcagc aacagcgttt tacaaggcac agccagtaat cgagtttgtt tgtgaagttt
      901 tggattttaa aagtattgaa gaacaacaaa aacctctgac agattcccaa agggtaaagt
      961 ttaccaaaga aattaaaggt ctaaaggtgg agataacgca ctgtgggca
//
