LOCUS       sequence-97865-e         619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 619)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MYO5AA-c010
FEATURES             Location/Qualifiers
     source          1..619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             13..35
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             90..122
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ctctagaata attttgttta actttaagaa ggagatatac catgggccat catcatcatc
       61 atcacgaaaa cctgtatttt cagggccata tggctagcat gactggtgga cagcaaatgg
      121 gtcgcggatc cgaattcatg gagaacatat ccccaggaca gatcattgat gaacccatcc
      181 gaccagtcaa cattcccagg aaagaaaagg atttccaagg gatgctggaa tacaagaagg
      241 aggatgagca aaaacttgtt aagaacctga ttctggaact gaagccacgt ggtgtagcag
      301 tcaatttgat tccaggatta ccggcatata tcctgttcat gtgtgttcga catgctgact
      361 acctgaatga tgatcagaaa gtaaggtcgt tgctaacatc aacaattaac agcatcaaaa
      421 aagtattgaa gaaaagaggt gatgattttg aaaccgtctc cttctggctc tctaacacat
      481 gccgattttt gcactgcttg aaacagtaca gtggagaaga gggctttatg aagcacaaca
      541 catctcgcca gaatgaacac tgcctcacca attttgacct ggctgagtat cggcaggtgc
      601 tgagtgactt ggccattca
//
