LOCUS       sequence-97868-e         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  TITLE     SGC_OxfordQ1Q2_2014
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DPYSL3A-c010
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             98..115
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             125..295
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             302..322
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tttcgtaaca gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc
       61 accatcgggc gcggatctcg gtccgaaaac catgggccac catcatcatc atcattcttc
      121 tggtgtggat aacaagttca acaaggagcg tcgaagagct cgccgtgaaa ttcgccatct
      181 gccgaacctg aaccgcgaac agcgtcgcgc atttattcgc agcctgcgcg atgatccgag
      241 ccagagcgcg aacctgctgg cggaagcgaa gaagctgaac gatgcgcagc cgaagggtac
      301 agagaacctg tacttccaat ccatgtccta ccaaggcaag aagaacatcc cgcggatcac
      361 gagtgaccgt ctccttatca agggaggcag aatcgtcaat gatgatcagt ccttttatgc
      421 tgatatttac atggaagatg gcttaataaa acaaattgga gacaatctga ttgttcctgg
      481 aggagtgaag accattgaag ccaatgggaa gatggtgatc cctggaggca tcgatgtcca
      541 tactcactac cagatgccat ataagggaat gaccacagta gatgacttct tccaagggac
      601 aaaggcggct ttagcaggtg gcaccaccat gatcattgac catgtggtgc ctgagcctga
      661 gtccagcctg actgaggcct atgagaaatg gagagagtgg gctgatggga agagttgctg
      721 tgactatgcc ctgcatgtgg acatcaccca ctggaatgac agcgtcaagc aggaagtgca
      781 gaacctcatc aaggacaaag gggttaactc cttcatggtt tatatggctt ataaggattt
      841 gtatcaagta tctaacacag agctctatga gatcttcacc tgcctgggag agctgggggc
      901 cattgctcaa gttcatgctg a
//
