LOCUS       sequence-97886-e        1030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP from jellyfish Aquorea sp. under T5 promoter with lac operators;
            ampicillin resistant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Siddall A, Williams AA, Sanders J, Denton JA, Madden D, Schollar J, 
            Bryk J
  TITLE     UNIGEMS: plasmids and parts to facilitate teaching on assembly, gene
            expression control and logic in E. coli
  JOURNAL   bioRxiv 2021.06.20.449138
REFERENCE   2  (bases 1 to 1030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.06.20.449138"
COMMENT     Sequence Label: p007ampGFP
FEATURES             Location/Qualifiers
     source          1..1030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(215..234)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(240..259)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        301..392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             577..955
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(611..630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggatgaacta tacaaataac cccaagggcg acacccccta attagcccgg gcgaaaggcc
       61 cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg
      121 gggagtcccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc
      181 aggtgggacc accgcgctac tgccgccagg caaacaaggg gtgttatgag ccatattcag
      241 gtataaatgg gctcgcgata atgttcagaa ttggttaatt ggttgtaaca ctgaccccta
      301 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      361 aaatgcttca ataatattga aaaaggaaga atatgagtat tcaacatttc cgtgtcgccc
      421 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      481 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      541 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      601 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      661 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      721 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      781 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      841 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      901 ccataccaaa cgacgagcgt gacaccccga tgcctgtagc gatggcaaca acgttgcgca
      961 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     1021 aggcggataa
//
